DISK RENEWAL IN RETINAL VISUAL CELLS

描述：这个应用程序探索了一个发现， 在光感受器中发现的生物钟是由一种机制调节的， 转录/翻译机制，并将输出转换为数字 有节奏行为的系统，包括转录一些 基因.为了探讨这些观察结果，有四个具体目标：1） 克隆和表征节律性mRNA，2）分析节律性mRNA的调控， 节奏信使RNA的光和多巴胺，以探索他们的 与生物钟的关系，3）以确定是否有节奏的基因产物 在其他脊椎动物视网膜中受到节律性调节， 其他生物钟系统和4）开发可转染的培养物 该系统适合于研究视网膜中的节律性基因表达。

DESCRIPTION: This application explores the discovery that a circadian clock found in photo receptors is regulated by a mechanism that includes a transcription/translation mechanism and provides output into a number of systems that show rhythmic behavior including transcription of some genes. To explore these observations, there are four specific aims: 1) to clone and characterize rhythmic mRNAs, 2) to analyze the regulation of rhythmic messenger RNAs by light and dopamine to explore their relationship to the clock, 3) to determine if the rhythmic gene products in Xenopus are rhythmically regulated in other vertebrate retinas or other circadian clock systems and 4) to develop a transfectable culture system suitable to study rhythmic gene expression in the retina.