GENETIC ANALYSIS OF THE DROSOPHILA ACK TYROSINE KINASE

果蝇蛋白Dock是在轴突生长锥中发现的衔接蛋白，其潜在地提供了引导信号受体与细胞骨架变化之间的联系。 dock null flies在四个定义的系统中经历轴突投射缺陷。Dock与果蝇胚胎和Schneider 2细胞中的p145相关。 从纯化的p145产生的蛋白质序列表明它属于活化的Cdc42hs酪氨酸激酶家族。 将完全克隆P145，并表征其与Dock的相互作用。 将利用局部P元件跳跃产生突变p145果蝇。 将分离多个p145等位基因，并将其指导表型与dock突变体中观察到的表型进行比较，以评估p145在轴突指导中的作用。

The Drosophila protein Dock is an adapter protein found in axonal growth cones that potentially provides a link between guidance cue receptors and changes in the cytoskeleton. dock null flies experience axonal projection defects in four defined systems. Dock associates with p145 in Drosophila embryos and Schneider 2 cells. Protein sequence generated from purified p145 indicates that it belongs to the family of Activated Cdc42hs tyrosine kinases. P145 will be fully cloned and its interaction with Dock will be characterized. Local P element hopping will be utilized to generate mutant p145 Drosophila. Multiple p145 alleles will be isolated and their guidance phenotypes will be compared to those seen in dock mutants to assess the role of p145 in axonal guidance.