GENETIC ANALYSIS OF MIDLINE PATTERNING IN ZEBRAFISH

形成图案的感应信令网络的性质 脊椎动物神经外胚层是发育生物学的中心问题。 下垫面对底板分化的诱导 Notochord是一个信号事件范例，它建立了 神经管的背腹轴。地板感应是中介的 Sonic Hedgehog，但关于其机制仍有许多未知之处 其中规定了楼板单元的命运。我建议去了解一下 基于细胞和分子的底板规范 斑马鱼(Danio Rerio)突变体独眼巨人(Cyc)的分析 删除底板和其他腹侧神经外胚层细胞类型。 这一提议将检验这样一种假设，即Cyc具有必要的 在地板规范中的作用，可能是调解感应 音速刺猬的行动..这项建议的目的是(1) 野生型和cyc突变胚胎中底板前体的特征 使用命运图谱技术，以及(2)分子鉴定环状病毒 基因的定位克隆。这些实验将提供关键的 有关野生型中线细胞类型规范的信息 胚胎，以及细胞周期蛋白在这一过程中所起的作用。

The nature of the inductive signaling networks that pattern the vertebrate neuroectoderm is a central problem in developmental biology. The induction of the floor plate differentiation by the underlying notochord is a paradigm of the signaling events that establish the dorsoventral axis of the neural tube. Floor plate induction is mediated by Sonic hedgehog, but much remains unknown about the mechanisms by which the floor plate cell fate is specified. I propose to learn about the basis of floor plate specification with a cellular and molecular analysis of the zebrafish (Danio rerio) mutant cyclops (cyc), which deletes the floor plate and other ventral neuroectodermal cell types. This proposal will test the hypothesis that cyc has an essential function in floor plate specification, perhaps mediating the inductive action of Sonic hedgehog.. The aims of this proposal are (1) to characterize floor plate precursors in wild-type and cyc mutant embryos using fate mapping techniques, and (2) to molecularly identify the cyc gene by positional cloning. These experiments will provide critical information about the specification of midline cell types in wild-type embryos, and the role that cyc plays in the process.