GENE REGULATION IN LENS REGENERATION

描述（改编自摘要）在一些尾尾动物中，晶状体切除术后，通过背虹膜色素上皮细胞（PECs）的转分化，新的晶状体再生。在培养中，腹侧PECs和其他动物的PECs都能转分化成晶状体。研究者建议使用这些系统来继续研究晶状体再生过程中的基因表达。到目前为止，他已经确定了几个基因，包括同源盒基因，成纤维细胞生长因子受体-1和Sonic hedgehog基因。他建议继续进行视网膜母细胞瘤和视黄酸受体的基因调控研究。他还建议研究从蝾螈的腹侧虹膜和不能再生晶状体的动物的背侧虹膜诱导晶状体再生。这将通过转染虹膜腹侧或背侧细胞中感兴趣的基因来完成。这些体内研究将由体外研究补充，以提供更准确的机制图。了解晶状体再生的诱导对于必须摘除晶状体的病理状态非常重要。

DESCRIPTION (adapted from the abstract)In some urodeles, following lentectomy a new lens regenerats by the transdifferentiation of dorsal iris pigment epithelial cells (PECs). In culture, ventral PECs as well as PECs from other animals are able to transdifferentiate into a lens. The investigator proposes to use these systems to continue his studies of gene expression during lens regeneration. He has thus far identified several genes including homeobox-containing genes, fibroblast growth factor receptor-1 and Sonic hedgehog. He proposes to continue with studies on gene regulation of retinoblastoma and retinoic acid receptors. He also proposes to study induction of lens regeneration from the ventral iris in newts and from the dorsal iris of animals incapable of lens regeneration. This will be done by transfecting the gene of interest in the ventral or dorsal iris cells. These in vivo studies will be supplemented by in vitro ones to provide a more accurate picture of the mechanisms. Understanding induction of lens regeneration would be of enormous importance in pathological states where the lens must be removed.