STRUCTURE DETERMINATION OF TUBULAR CRYSTALS

本文的研究目的是发展钢管的分析方法， 结构转换成标准的电子晶体学方法， （1）解决烟碱乙酰胆碱受体的原子结构， 分辨率和（2）从任何管求解三维结构 以半自动化的方式进行标本采集。 为了实现目标1，将对图像处理例程进行改进 将分辨率扩展到目前的4.6A以上。领域包括更准确 测量和校正失真，精细化处理，以占 重叠贝塞尔项的噪声和埃瓦尔德球的修正 曲率、光束倾斜和对比度传递函数。 目标2将通过分析所研究的蛋白质管状晶体来实现 其他研究人员使用为乙酰胆碱建立的方法 自动化后的接收器。图像的严谨性 处理和广泛适用的自动化方法将是 测定

The proposed research is to develop the analysis of tubular structures into a standard electron crystallographic approach that can be used to (1) solve the structure of the nicotinic acetylcholine receptor at atomic resolution and (2) solve the three-dimensional structure from any tubular specimen in a semi-automated approach. To accomplish aim 1, improvements in the image processing routines will be made to extend resolution beyond the current 4.6A. Areas include more accurate measurement and correction of distortions, refined treatment to account for noise from overlapping Bessel terms, and corrections for Ewald sphere curvature, beam tilt and the contrast transfer function. Aim 2 will be achieved by analyzing tubular crystals of proteins being studied by other investigators using the methods established for the acetylcholine receptor after they have been automated. The rigorousness of the image processing and the broad applicability of the automated methodology will be determined.