OXYGENASE METABOLITES AND RENAL VASCULAR ACTIVITY

描述：(改编自应用程序)总体假设为 在这项提议中检验的是增加脂氧合酶的产生 代谢物和环氧合酶代谢物的减少 有助于增强肾小球前血管的张力和抬高 与循环血糖素水平升高相关的血压 血管紧张素II。大鼠将用血管紧张素慢性输注制备 II和高血压的发展随之而来。在选定的时间 每隔一段时间，将测量环氧合酶和脂氧合酶的代谢产物。 将评估和分析肾微血管的反应性。 花生四烯酸的各种代谢物的产生。此外, 体外延髓旁肾单位技术与视频显微镜的结合 将用于在体外确定肾小球前血管张力；各种不同的 将评估代谢物脂氧合酶和环氧合酶的贡献 使用这些探针的药物抑制在急性和非传染性疾病中 长期的状况。这些研究的结果将进一步定义 肾素-血管紧张素系统与花生四烯酸的作用 血管紧张素依赖性高血压发病过程中的代谢。

DESCRIPTION: (Adapted from the application) The overall hypothesis to be tested in this proposal is that increases in production of lipoxygenase metabolites and decreases in production of epoxygenase metabolites contribute to enhanced vascular tone of preglomerular vessels and elevation in blood pressure associated with increased circulating levels of angiotensin II. Rats will be prepared with chronic infusion of angiotensin II and the development of hypertension followed. At selected time intervals, the metabolites of epoxygenase and lipoxygenase will be measured. The reactivity of renal microvasculatures will be evaluated and assayed for the production of the various metabolites of arachidonic acid. In addition, in vitro juxtamedullary nephron techniques combined with video microscopy will be used to determine preglomerular vascular tone in vitro; the various contributions of metabolites lipoxygenase and epoxygenase will be assessed using pharmacological inhibition of these probes both in the acute and chronic situation. Results of these studies will further define the renin-angiotensin system and determine the role of arachidonic acid metabolism in the development of angiotensin-dependent hypertension.