QUANTITATION OF CARDIAC MYOCYTE CONTRACTILE DYNAMICS

许多基本问题都是为了理解生理学 尤其是，心肌的病理生理学现在 使用分离的心肌细胞在细胞水平上进行处理，或 肌细胞。此应用程序的主要目标是开发简单的 和经济的数字成像工具，心肌研究 社区可以用来记录心肌细胞的生物力学特性 在肌肉收缩时。该项目将分三个阶段进行 发展的具体目标：(1)高速率数字图像 收缩心肌细胞可视化采集(2) 基于软件的心肌细胞长度记录实时执行 分析数字图像数据以动态表征、显示和 在线保存心肌细胞长度数据，(3)肌节 长度记录是对高速图像采集方法的扩展 以及实时量化肌节长度的数值分析 在肌肉收缩时。这些方法从根本上改进了 现有技术在功能能力和简单性方面 使用。以商业化形式设想的仪器系统有 也很经济，因此更多感兴趣的实验室可以使用 在细胞水平上寻求心肌研究问题。

Many of the fundamental questions aimed at understanding the physiology and, particularly, the pathophysiology of the myocardium are now being addressed at the cellular level using isolated cardiac muscle cells, or myocytes. The principal aim of this application is to develop simple and economical digital imaging tools that the cardiac muscle research community can use to record the biomechanical properties of myocytes during muscle contraction. The project will be undertaken as three Specific Aims for the development of: (1) High-Rate Digital Image Acquisition for visualization the contracting cardiac myocyte, (2) Software-Based Myocyte Cell Length Recording to perform real-time analysis of digital image data to dynamically characterize, display and save the cardiac myocyte cell length data on-line, and (3) Sarcomere Length Recording expands on the methods of high-rate image acquisition and numerical analysis to quantify the sarcomere length in real-time during muscle contraction. These methods fundamentally improve on existing techniques in terms of functional capability and simplicity of use. In commercialized form the instrumentation systems envisioned are also economical and thus accessible to many more laboratories interested in pursuing cardiac muscle research questions at the cellular level.