FLP PROTEIN COMPLEXED W/ DNA

FLP是lambda整合酶家族的一种位点特异性重组酶。 它与特定的DNA位点结合，然后切断DNA链并 在重组配置中使用它们，如下所示： 反应机理与Cre重组酶(1)非常相似， 这两种蛋白质之间的序列同源性极其有限。FLP (来自酵母)是这个家族中唯一的真核成员，并且是 预期在几个有趣的方面与cre有所不同，包括 在组装活动站点时使用域交换。这个 复合反应不需要辅因子，而且反应的能量 DNA骨架的磷酸二酯键以共价形式储存 磷酸酪氨酸中间体。数据收集在BioCARS站上 14-BM-D

Flp is a site-specific recombinase of the lambda integrase family. It binds to specific DNA sites, then cuts the DNA strands and religates them in recombinant configuration, as below: Although the reaction mechanism is very similar to that of Cre recombinase (1), the sequence homology between the two proteins is extremely limited. Flp (from yeast) is the only eukaryotic member of this family and is expected to differ from cre in several intersting aspects, including the use of domain swapping in assembling the active site. The recombination reaction requires no cofactors, and the energy of the phosphodiester bonds of the DNA backbone is stored in a covalent phospo-tyrosine intermediate. Data was collected on BioCARS Station 14-BM-D.