CORE--FLUORESCENT IN SITU HYBRIDIZATION/CYTOGENETICS

该核心的主要目标是：1）比较 间期FISH、超中期FISH和RT-PCR在其 预测预后、监测治疗反应的能力，以及 预测复发，2）支持所需的基因图谱研究 Lalitha Nagarajan博士来定义 7号染色体，负责7 q的不良预后- 患者 该核心将执行1）间期核FISH，以检测 染色体易位的白血病细胞，如t（15;17） 和t（9;22）; 2）开发inv（16）和t（8;21）的间期探针 与Siciliano、克拉克斯顿和Chang博士合作， 开发5 q和7 q的间期探针，与 博士Nagarajan将iFISH结果与超中期FISH相关联 (HMF)和RT-PCR来支持基因所需的FISH研究 在7号染色体上定位，以确定这一关键区域， 染色体是导致预后不良的原因（Lalitha博士 Nagarajan）。 该核心将与Elihu Estey博士合作， 反应，理查德·尚普林博士监测对BMT的反应， 克里斯托弗阅读富集二倍体干细胞，相关iFISH， HMF和RT-PCR，以及Lalitha Nagarajan博士绘制了 7号染色体上的区域是导致预后不良的原因。

The major goals of this core are: 1) to compare the utilities of interphase FISH, hypermetaphase FISH, and RT-PCR in their abilities to predict prognosis, monitor therapy responses, and predict relapse, and 2) to support the gene mapping studies needed for Dr. Lalitha Nagarajan to define the critical regions on chromosome 7 that are responsible for poor prognosis of 7q- patients. This core will perform 1) FISH on interphase nuclei to detect leukemic cells with chromosomal translocations such as t(15;17) and t(9;22), 2) develop interphase probes for inv (16) and t(8;21) in collaboration with Drs. Siciliano, Claxton and Chang, and develop interphase probes for 5q- and 7q- in collaboration with Dr. Nagarajan to correlate iFISH results with hypermetaphase FISH (HMF) and RT-PCR to support the FISH studies needed for gene mapping on chromosome 7 to define the critical regions on this chromosome that are responsible for poor prognosis (Dr. Lalitha Nagarajan). This core will collaborate with Dr. Elihu Estey to monitor therapy responses, Dr. Richard Champlin to monitor responses to BMT, Dr. Christopher Reading to enrich diploid stem cells, correlate iFISH, HMF, and RT-PCR, and Dr. Lalitha Nagarajan to map the critical regions on chromosome 7 that are responsible for poor prognosis.