2- AND 3-D IMAGING OF METABOLIC ACTIVITION OF BETA CELLS

速冻胰腺的高分辨率（50 mu）二维和三维成像在可行性研究中显示了响应于葡萄糖挑战的来自NADH（P）H和黄素蛋白（Fp）减少和血红蛋白浓度增加的独特局部信号。 该结果鼓励比较响应于葡萄糖和亮氨酸滴定的图像，以区分线粒体和胞质NADH（P）H的响应。 氧化还原变化的时间分辨成像提供了进一步的区别;预计胞质响应将先于线粒体（Fp）响应，这些时间分离将通过葡萄糖/亮氨酸比较来确认。 将通过对组织块进行最后一次光学扫描后处理和切片的组织切片进行组织病理学检查，确认β细胞反应的鉴定。 预计这些快速冷冻成像研究将为基于方法2（飞行荧光）和方法3（NIR经腹成像）的体内β细胞功能的未来微创光学评价提供依据。

High resolution (50 mu)2- and 3-dimensional imaging of snap frozen pancreas shows in feasibility studies distinctive localized signals from NADH(P)H and flavoprotein (Fp) reduction and hemoglobin concentration increases in response to glucose challenges. This result encourages comparison of images in response to titrations with glucose and leucine in order to differentiate the response of mitochondrial and cytosolic NADH(P)H. A further distinction is afforded by time-resolved imaging of redox changes; it is expected that cytosolic responses will precede mitochondrial (Fp) responses and these time separations will be confirmed by glucose/leucine comparisons. Identification of beta cell responses will be confirmed by histopathology of tissue sections processed and microtomed from the tissue block which the last optical scan is made. It is expected that these snap frozen imaging studies will afford a rationale for future minimally invasive optical evaluation of beta cell function in vivo base upon Method 2, Flying Fluorescence and Methods 3, NIR Transabdominal Imaging.