MICROSATELLITES AS MARKERS FOR BLADDER CANCER

高通量微卫星分析鉴定肿瘤特异性遗传 与膀胱癌进展相关的改变。拟议工作 旨在探讨与膀胱癌管理相关的几个临床问题， 基于原发性肿瘤中这些遗传变化的检测， 尿该项目的具体目标是：（1）确定 敏感性和特异性的微卫星标记面板为 复发性浅表性膀胱癌的检测，以及（2） 初步探讨微卫星的特定模式是否 改变（即，洛缺失或不稳定性）与以下风险增加相关： 浅表和浸润性疾病复发或进展 癌的这些具体目标将通过前瞻性的 对来自以下患者的配对肿瘤和尿液标本进行微卫星分析： 接受治疗的浅表性和浸润性膀胱癌患者 在约翰霍普金斯医院接受治疗分析将是 使用项目#1中开发的分子方法和技术进行 和2.这个项目更大的目标是奠定一个实践基础 为今后开展监测微型卫星分析研究和 为不同阶段的病人建立风险概况 膀胱癌

High throughput microsatellite analysis identifies tumor-specific genetic alterations associated with bladder cancer progression. The proposed work seeks to explore several clinical issues related to bladder cancer managed based on the detection of these genetic changes in primary tumors and urine. The specific aims of this project are: (1) to define the sensitivity and specificity of microsatellite marker panels for the detection of recurrent superficial bladder cancers and, (2) to preliminarily explore whether specific patterns for microsatellite alterations (i.e., LOH or instability) correlate with increased risk of disease recurrence or progression in both superficial and invasive cancers. These specific aims will be accomplished through prospective microsatellite analysis of paired tumor and urine specimens obtained from patients with superficial and invasive bladder cancers undergoing therapy with curative intent at the John Hopkins Hospital. The analyses will be performed using molecular methods and technology developed in projects #1 and 2. The larger goal of this project is to lay a practical foundation for future studies of microsatellite analysis for monitoring and for the establishment of risk profiles for patients with different stages of bladder cancer.