MARINE AND FRESHWATER BIOMEDICAL SCIENCES CENTER

这项提议是为了继续为国家科学研究所提供资金 环境健康科学海洋和淡水生物医学科学 迈阿密大学研究中心。位于罗森斯蒂尔学校 海洋和大气科学园区，该中心是一个协作性的 10名中心调查人员、6名研究调查人员和5名附属人员的努力 中心调查人员。三个委员会监督中心的运作： 执行委员会、内部咨询委员会和外部 咨询委员会。两个主要的研究主题构成了 跨学科中心工作：海洋毒素和饮食风险，以及海洋 人类疾病模型系统。这两个研究主题都被考虑在内。 对迈阿密大学MFBS中心的使命至关重要，而且两者 都在NIEHS赞助的研究范围内。第一个研究主题 包括毒素生物合成、新陈代谢、分子药理学、分子生物学 模特儿。电生理学、孤儿受体和江湖骗子调节剂， 分离技术和检测方法、流行病学和公共卫生 健康。第二个研究主题包括六个不同类型的海洋模型 发育阶段；幼稚的神经纤维瘤病。黄鱼肝 代谢，鲨鱼免疫学，海兔生物学，转基因鱼类，以及 哨兵物种。已经选择了四个试点项目，这些项目提供了新的 在明确的研究主题范围内的研究方向；“免疫系统 《大西洋宽吻海豚的特征》，《作为模式的海豚》 发育神经毒理学系统“，以及”J链和 鲨鱼免疫球蛋白生物合成中的分泌尾“，以及 “电压敏感钠通道的计算化学：毒素” 效果。 作为对现有个人和协作方案的支持4 根据利用率和总体情况提出设施和服务核心 在中心运营的1-4年中对各个项目的价值；毒素 提供短杆菌毒素、岩藻毒素、冈田酸、软骨藻酸和 雪卡毒素及其应用相关毒素衍生物的体外和体内研究 提供组织培养和整体动物服务的活体检测系统 使用电子显微镜、遗传结构、计算机的方法 成像，以及原代和转化的海洋细胞类型。电生理学 该公司提供两个独立的设备齐全的钻井平台，用于毒素工作和海洋 模型研究和鱼类和贝类养殖提供个人 对模型系统的标本进行研究。一个定义明确的社区 外展和教育方案将继续，并执行一项 毒物控制中心将承担海鲜中毒的热扣留 在迈阿密毒药控制中心工作。迈阿密大学将 继续以费用分担的形式对中心作出承诺。 大学的捐款将用于资助博士后研究 员工及其差旅和会议费用，以帮助支持两个 年轻教员将担任电生理学和 为中心主任提供参加中心主任会议及相关会议的差旅 费用。 该方案在6-10年的总体目标将是继续 传播NIEHS信息，开创人类基础科学研究的先河 与天然毒物有关的环境健康问题，并将继续 寻找和表征环境诱导的新海洋模型 人类疾病。

This proposal is to provide continued funding of a National Institute of Environmental health Sciences Marine and Freshwater Biomedical Sciences Center at the university of Miami. Housed on the Rosenstiel School of Marine and Atmospheric Sciences campus, the Center is a collaborative effort of 10 Center Investigators, 6 Research Investigators, and 5 Adjunct Center Investigators. Three committees oversee Center operation: The Executive Committee, the Internal Advisory Committee, and the External Advisory Committee. Two principal Research Themes form the basis for the interdisciplinary Center work: Marine Toxins and Dietary Risk, and Marine Model Systems of Human Diseases. Both research themes are considered essential to the mission of the University of Miami MFBS Center, and both are within the scope of NIEHS-sponsored research. The first research theme includes Toxin biosynthesis, metabolism, Molecular Pharmacology, Molecular Modeling. Electrophysiology, Orphan Receptors and Charlatan Regulators, Separation Techniques and Assay Methods, and Epidemiology and public Health. The second research theme includes six marine models in various stages of development; Damselfish Neurofibromatosis. Taodfish Hepatic metabolism, Shark Immunology, Aplysia Biology, Transgenic Fishes, and Sentinel Species. Four Pilot Projects have been selected which provide new research directions within the defined research themes; "Immune System Characterization of Atlantic Bottlenose Dolphins", "Aplysia as a Model System for Developmental Neurotoxicology", and "Function of the J-Chain and the Secretory Tail in Immunoglobulin Biosynthesis int he Shark", and "Computational Chemistry of Voltage-Sensitive Sodium Channels: Toxin Effects. As support for the existing individual and collaborative programs 4 Facilities and Service Cores are proposed, based on utilization and overall value to individual programs in Years 1-4 of Center operation; Toxin Probes supplying brevetoxins, saxitoxin, okadaic acid, domoic acid and ciguatoxins and application-related toxin derivatives, In Vitro and in In Vivo Assay Systems which provides a service tissue culture and whole animal approach employing electron microscopy, genetic constructs, computer imaging, and primary and transformed marine cell types. Electrophysiology which provides two separate fully-equipped rigs for toxin work and marine model studies, and Fish and Shellfish Cultivation which provides individual specimens of the model systems for study. A well-defined Community Outreach and Education Program will continue, and implementation of a poison-control center hot lien for seafood intoxication will be undertaken with the Miami Poison Control Center. The University of Miami will continue its commitment to the Center in the form of cost-share. University contributions will be used to fund post-doctoral research associates and their travel and meeting costs, to aid in support of two young faculty who shall act as co-core leaders in Electrophysiology and to provide Center Director travel to Center Director meetings and related expenses. The overall goals of the program in years 6-10 will be to continue to communicate the NIEHS message, to pioneer basic science approaches to human environmental health problems related to natural toxicants, and to continue to search out and characterize new marine models of environment-induced human disease.