ABI PRISM 7700 SEQUENCE DETECTION SYSTEM

这是一项资金申请，用于购买ABI PRISM 7700序列检测系统，供NIH资助的三名临床研究人员使用，以监测登记参加基因转移研究的患者。与传统的半定量聚合酶链式反应相比，使用该机器的实时定量聚合酶链式反应检测DNA或RNA具有几个优点：提高了灵敏度和特异度，缩短了分析时间，并通过消除对放射性探查的要求而增加了安全性。因此，研究人员在本申请中描述的项目将能够以更高的准确性和便利性解决转基因表达的量化问题，提高安全性并增加从临床研究研究中获得的信息。

This is an application for funds to purchase an ABI PRISM 7700 Sequence Detection System for use by three NIH funded clinical researchers to monitor patients enrolled in gene transfer studies. Detection of DNA or RNA by real time PCR using this machine has several advantages over conventional semi-quantitative PCR in providing increases sensitivity and specificity as well as reducing the time of the assay and increasing safety by eliminating the requirement for radioactive probing. The projects described by the investigators within this application would therefore be able to address issues of quantitation of transgene expression with a much higher degree of accuracy and facility, improving the safety and increasing the information to be gained from the clinical research studies.