PURCHASE OF A MALDI-TOF MASS SPECTROMETER

为购买基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)提供支持，这是许多NIH资助的研究项目所必需的。加州大学欧文分校不提供MALDI-TOF-MS。目前的研究由于缺乏MALDI-TOF系统而受到阻碍。在借用申请仪器期间取得的生产率提高和所获得的经验证明了这一点，这对UCI的三个学院：物理科学、生物科学和医学至关重要，因此将真正成为一个共享的跨学科工具。主要用户的研究项目包括：(A)Shea，新型聚合物、共聚物、嵌段聚合物和离聚体，(B)Chamberlin，确定神经元谷氨酸受体的结合位点；(C)Feher，在倍半硅氧烷核心上具有肽和碳水化合物功能的新型树枝状化合物；(D)用于阿尔茨海默病研究的Glabe、淀粉样蛋白和多肽结构表征；(E)Henschen，关于纤维蛋白原正常形式和变体的结构/功能研究；(F)Selsted，新型多肽抗菌剂的表征和活性；(G)Van Vranken，研究蛋白质衰老过程中的化学过程。除了主要的用户群体，还有许多其他由美国国立卫生研究院资助的研究小组迫切需要MALDI-TOF能力。要购买的仪器的性质是，研究人员可以很容易地操作它，但也可以通过将其放置在具有20多年质谱学经验的设施主任的质谱学设施中来充分利用它。由两台磁扇区仪器组成的UCI质谱仪已完全饱和，为合成程序(萃取质量测定)和其他问题的解决提供分析支持，特别是使用LSIMS、直接探针、GC-MS和FD。这两台磁力部门仪器每年的样本量超过3,500个，其中绝大多数需要进行萃取质量测定。UCI对质谱学设施以及其他支持研究人员的分析光谱学仪器设施有长期的重大资金承诺。目前，每年对这些设施的8项薪金和业务支助承诺超过500 000美元。额外的运营资金是通过向用户社区的赠款和联系人收取费用来获得的。

Support for purchasing matrix assisted laser desorption ionization time of flight mass spectrometer (MALDI-TOF-MS) which is essential to many NIH-funded research programs is requested. No MALDI-TOF-MS is available on the UC Irvine campus. Current research is being hindered by the lack of a MALDI-TOF system. This was demonstrated by the productivity gains that were made, and the experience gained, during the loan of a requested instrument is vital to the interests of three Schools at UCI: Physical Sciences, Biological Sciences and Medicine and will therefore be truly a shared inter-disciplinary instrument. Research projects of the major users include: (a) Shea, novel polymers, co-polymers, block polymers and ionomers, (b) Chamberlin, binding site determination for neuronal glutamate receptors; (c) Feher, novel dendritic compounds with peptidic and carbohydrate functionalities on a silsesquioxane core; (d) Glabe, amyloid protein and peptide structural characterization for Alzheimer's disease research; (e) Henschen, structure/function studies on normal and variant forms of fibrinogen; (f) Selsted, characterization and activity of novel peptidic antimicrobial agents; (g) Van Vranken, studies on chemical processes in protein aging. Aside from the major user group there are many other NIH funded research group who urgently require MALDI-TOF capability. The nature of the instrument to be purchased is such that it can be readily operated by the researcher but also that it will be fully exploited by placing it in a mass spectrometry facility with facility director who has over 20 years experience in mass spectrometry. The UCI mass spectrometry facility which comprises of two magnetic sector instruments is fully saturated providing analytical support for synthetic program (extract mass determinations) and other problem solving, particularly using LSIMS, direct probe, GC-MS and FD. The sample load for the two magnetic sector instruments is over 3,500 per year with the vast majority requiring extract mass determinations. UCI has a long-term major funding commitment to the mass spectrometry facility as well as the other analytical spectroscopy instrumentation facilities which support the research faculty. Currently the annual commitment is over $500,000 to these facilities in eight salaries and operational support. Additional operation funding is obtained through charges to the grants and contacts of the user community.