UPGRADE OF NMR FACILITIES

本申请是维克森林大学医学院的研究人员提出的一项要求，即通过在设施中添加AVANCE 300 NMR光谱仪来升级我们的NMR设施。AVANCE控制台将连接到新的标准孔（52 mm）磁体系统和我们的老布鲁克AM 300 MHz超宽孔（98 mm）磁体。旧控制台的匀场控制将保留，用于匀场超宽孔磁体。新的核磁共振将给出更小分子的更好质量的核磁共振谱，即，质量小于2,000 amu的分子。此外，将提供定量信息的生物材料的数量，产生的各种代谢过程中使用13 C富集示踪剂。目前的控制台，一个AM-300超宽口径控制台与Aspect 3000计算机，是13岁，并开始显示其年龄。较新的游戏机有更好的电子和计算机，较新的软件更灵活和用户友好。新的控制台和磁性将为一维NMR和二维COSY、NOESY、HC-COSY、HSQC和HMQC实验提供1）更高的可靠性、2）更高的灵敏度和3）更好的分辨率。保留大口径磁铁将允许用户继续对灌注器官和小动物进行核磁共振研究。

This application is a request from investigators at the Wake Forest University School of Medicine to upgrade our nmr facilities by adding an AVANCE 300 NMR spectrometer to the facility. The AVANCE console will be interfaced to a new standard bore (52 mm) magnet system and to our older Bruker AM 300 MHz superwide bore (98 mm) magnet. The shim controls from the old console will be retained for shimming the superwide bore magnet. The new nmr will give better quality nmr spectra for smaller molecules, i.e., molecules with masses less than 2,000 amu. In addition, will will provide quantiative information on the amounts of biomaterials that produced by various metabolic processes using 13C-enriched tracers. The present console, an AM-300 superwide bore console with an ASPECT 3000 computer, is 13 years old and starting to show its age. Newer consoles have much improved electronics and computers and the newer software is more flexible and user friendly. A new console and magnetic will provide 1) increased reliability, 2) higher sensitivity, and 3) better resolution, for 1-dimensional nmr and 2-dimensional COSY, NOESY, HC-COSY, and HSQC, and HMQC experiments. Retaining the larger bore magnet will allow users to continue nmr studies on perfused organs and small animals.