BIOMOLECULAR IMAGING SYSTEM

这项共享仪器拨款申请的目的是获得NCRR资金，用于购买一套分析成像系统，由维克森林大学医学院比较医学临床研究中心的一组NIH资助的研究人员共享。主要用户组由来自4个独立NIH研究所的2个计划项目和2个RO1拨款支持。为了有效地推进他们的研究，这些研究人员对敏感的、定量的、分子分析有关键的需求。所要求的仪器是一台分子动力学STORM 860 PDSI分析成像系统，这是一种独特的系统，具有存储荧光粉放射自显影（磷光成像）、直接荧光分析和化学荧光分析的能力。这些特性有助于在核酸和蛋白质分析中进行广泛的分析分析。该仪器具有宽的线性动态范围，适用于任何曝光强度下的定量数据收集，消除了重复扫描和可变曝光时间的需要。其他功能包括高分辨率图像，短扫描时间，大格式能力和强大的软件，以快速提供准确的数据。该设施目前没有能够执行任何这些功能的类似设备。仪器的现场可用性将大大扩展主要用户组的分析能力，一旦可用，将吸引更多的用户和应用程序。该系统将使我们能够在现场进行所有的分析，这将提高研究者在研究各种疾病过程中涉及的机制方面的生产力和效率。维克森林大学医学院的比较医学部门完全支持拟议的计划，并将在必要时为仪器的维护提供空间和持续支持，从而为这项工作做出贡献。

The purpose of this shared instrument grant application is to obtain NCRR funds to purchase an analytical imaging system to be shared by a group of NIH funded investigators at the Comparative Medicine Clinical Research Center of the Wake Forest University School of Medicine. The Major User Group is supported by 2 Program Projects and 2 RO1 grants from 4 separate NIH institutes. These investigators have critical needs for sensitive, quantitative, molecular analyses in order to effective advance their research. The instrumentation requested is a Molecular Dynamics STORM 860 PDSI Analytical Imaging System, a unique system which has capability of storage phosphor autoradiography (phosphorimaging), direct fluorescence analysis, and chemifluorescence analysis. These characteristics facilitate a broad range of analytical assays useful in nucleic acid and protein analysis. The instrument has a wide, linear dynamic range useful for quantitative data collection at any exposure intensity, eliminating the need for repeated scans and variable exposure times. Other features include high-resolution images, short scan times, large format capability, and powerful software to rapidly provide accurate data. No comparable equipment capable of performing any of these functions currently exists at this facility. The on site availability of the instrumentation will greatly expand the analytical capabilities of the Major User Group and will attract more users and applications once available. This system will enable us to carry out all of our analyses on site, which will increase investigator productivity and efficiency in the investigation of mechanisms involved in a variety of disease processes. The Section on Comparative Medicine of the Wake Forest University School of Medicine fully supports the proposed plan, and will contribute to the effort by providing space and continuing support for the maintenance of the instrumentation as necessary.