ABI PRISM MODEL 377 DNA SEQUENCER

ABI Prism 377自动DNA测序仪的申请是由NIH支持的一组研究人员提交的，他们使用DNA测序作为他们研究工作的重要组成部分。他们代表了一个由128名主要研究者组成的基础广泛的用户群体，其中大多数是NIH资助人，他们的研究主要依赖于成本效益和成本效益的DNA测序，以表征自然种群、诱导和自发突变体以及实验室构建的DNA变异和突变。应用于基础和应用生物医学研究，包括对人类、狗、老鼠、果蝇、酵母、线虫、病毒和植物的研究。目前，一个ABI 377自动DNA测序仪可通过伊萨卡的DNA测序通用设施获得，服务于伊萨卡校区和纽约市医学院。它目前的容量为81%（每月2600多个序列），按照目前的增长速度，将在三个月内（到1998年6月）达到最大容量。这就出现了两个问题。当产能达到时，周转时间将会增加，从而推迟研究。其次，在机器停机的时候，所有的DNA测序都停止了。为了缓解这一情况，康奈尔大学生物资源中心需要第二台ABI Prism 377 DNA测序仪，以使其更接近高通量DNA测序和基因分型的适当标准，为这一庞大的研究人员群体提供服务。

This application for an ABI Prism 377 Automated DNA Sequencer is being submitted by a group of NIH supported investigators who use DNA sequencing as a significant part of their research effort. They represent a broad-based user group of 128 principal investigators, the majority of which are NIH grantees, whose research depends critically on cost efficient and cost effective DNA sequencing to characterize DNA variation and mutations in natural populations, induced and spontaneous mutants, nad laboratory constructs. Applications are in basic and applied biomedical research including work on humans, dogs, mice, Drosophila, yeast, nematodes, viruses, and plants. Currently, a single ABI 377 automated DNA sequencer is available through a common-use facility for DNA sequencing in Ithaca, serving both the Ithaca campus and the NY City Medical College. It is currently at 81% capacity (more than 2600 sequences per month), and at current rate of growth, will be at maximum capacity within three months (by June 1998). This presents two problems. As capacity is reached, turn-around time will increase, delaying research. Second, in times of machine down-time, all DNA sequencing is shut down. A second ABI Prism 377 DNA Sequencer is requested for this facility to alleviate this situation and move Cornell's BioResource Center closer to an appropriate standard for high throughput DNA sequencing and genotyping for this large group of investigators.