SUBCELLULAR STRUCT OF PHAEOCYSTIS & ITS RELATION TO ITS ABSORPTION PROPERTIES

兰尼定受体是一种钙释放通道，定位于 肌肉和神经元的内膜系统。三种异构体 兰尼定受体在鸡中已被描述：两个骨骼 肌肉形态称为阿尔法和贝塔，还有一种心肌形态。这个 兰尼定受体在发育过程中的分布 用两种单抗(Mab)制备鸡脑抗体。Mab110F 独家识别出阿尔法骨骼肌形态和Mab110E 同时识别贝塔和心脏形式，但不识别阿尔法形式。在……里面 发育中的成年鸡脑，只有小脑浦肯野脑 神经元含有α-兰尼定受体，而神经元贯穿于 大脑的其余部分包含贝塔/心脏形式。在成体中， 几乎没有看到贝塔/心脏形式的免疫标记 浦肯野神经元。然而，贝塔/心脏形态的分布 在出生后的第一周内发生了变化。而在胚胎时期 小脑，贝塔/心脏形态主要定位于 浦肯野神经元，浦肯野神经元内的标记量 在出生后第一周内逐渐减少，而罚款， 分子层开始出现点状标记。至 确定这些轮廓是否真的是突触前突触 在平行纤维中，切开1-2微米厚的小脑切片。 并在400keV电压下用IVEM检查。在这 方向，平行的纤维在纵向上运行 方向。使用IVEM，可以看到点状标记是 与静脉曲张相关，包含在纵向延伸的 与平行纤维大小和形态相同的纤维，提供 进一步的证据表明，贝塔/心脏形式位于 平行纤维的突触前突触。这是第一批 神经末梢含有钙的解剖学证明 能够从细胞内储存的钙动员起来的通道。 这项研究构成了欧阳博士博士论文的一部分。一个 详细描述这一工作的手稿发表在欧阳等人的期刊上。脑区 研究报告，775：52-62,1997。

The ryanodine receptor is a calcium release channel localized to the internal membrane system in muscle and neurons. Three isoforms of the ryanodine receptor have been described in chicken: two skeletal muscle forms termed alpha and beta and a cardiac muscle form. The distribution of the ryanodine receptor was mapped in developing chicken brain using two monoclonal antibodies (Mab). Mab110F recognized the alpha skeletal muscle form exclusively and Mab110E recognized both the beta and cardiac forms but not the alpha form. In the developing and adult chicken brain, only cerebellar Purkinje neurons contain the alpha ryanodine receptor while neurons throughout the rest of the brain contain the beta/cardiac form. In the adult, little immunolabeling for the beta/cardiac form was seen within Purkinje neurons. However, the distribution of the beta/cardiac form changed during the first post-natal week. Whereas in the embryonic cerebellum, the beta/cardiac form was localized prim arily within Purkinje neurons, the amount of labeling within Purkinje neurons gradually decreased during the first post-natal week while a fine, punctate labeling began to appear in the molecular layer. To determine whether these profiles were indeed the presynaptic boutons of parallel fibers, 1-2 um thick sections of cerebellum were cut in the coronal orientation and examined at 400 keV using IVEM. In this orientation, the parallel fibers are running in a longitudinal direction. Using IVEM, the punctate labeling was seen to be associated with varicosites contained within longitudinal stretches of fibers of the same size and morphology as parallel fibers, providing further evidence that the beta/cardiac form is localized within presynaptic boutons of parallel fibers. This was one of the first anatomical demonstrations that nerve terminals contain calcium channels capable of mobilizing calcium from intracellular stores. This research formed part of Dr. Ouyang's doctoral dissertation. A manuscript detailing this work was published in Ouyang et al. Brain Res., 775: 52-62, 1997.