MEMBRANOUS COPPER AMINE OXIDASE

产品描述铜胺氧化酶（CAO）是将内源性胺转化为氨、过氧化氢和醛的酶家族。迄今为止，在血清型和膜结合型两种CAO中，膜型CAO的研究尚不深入。我们打算获得先前分离的克隆膜CAO cDNA，以表达的酶在哺乳动物细胞和表征其动力学特性，辅因子的形成和催化机制。鉴于CAO在几种疾病中的潜在重要性，我们打算通过组合方法合成肼基抑制剂。我们希望这些化合物的筛选将产生对膜结合CAO特异性的抑制剂。我们还打算研究可能的作用，膜CAO作为发电机的过氧化氢在质膜。过氧化物的缓慢原位生成可能会充当信号传导剂，从而影响负责细胞死亡、细胞增殖和炎症的基因。

DESCRIPTION Copper amine oxidases (CAOs) are a family of enzymes that convert endogenous amines to ammonia, hydrogen peroxide and aldehydes. To date, of t5he two types of CAOs, serum and membrane bound, the membranous CAOs have not been thoroughly studied. We intend to obtain the previously isolated clone for membranous CAO cDNA in order to express the enzyme in mammalian cells and characterize its kinetic properties, cofactor formation and catalytic mechanism. Given that CAOs are potential important in several diseases, we intend to synthesize hydrazine-based inhibitors by combinatorial methods. It is our hope that screening of these compounds will yield an inhibitor specific for membrane bound CAOs. We also intend to investigate the possible role of membranous CAO as a generator of H2O2 at the plasma membrane. It is possible that the slow, in situ generation of peroxide will act as a signaling agent, thereby influencing the genes responsible for cell death, cell proliferation and inflammation.