STABALIZED LIPID PLATFORMS FOR PROTEIN IMMOBOLIZATION

本研究的目标是制备和表征新型聚合物磷脂双层组装体，作为蛋白质附着的底物。将蛋白质受体固定在双层膜表面，对于利用结合蛋白作为生物选择性识别元件开发新型生物传感器具有重要的实际应用价值。实现脂双层作为蛋白质固定化的底物并随后用作生物传感器需要改善其化学和结构特性，同时保持脂双层的低非特异性结合特性。这里要研究的策略是磷脂双层的侧向聚合与蛋白质在表面的定点固定相结合。拟开展的研究包括新型系链平面聚合物磷脂双层膜的制备和表征。然后将通过溶解度和光学测量来评估所制备的交联型聚合物磷脂双层膜的稳定性。聚合物脂双层随后将被用作定点定向蛋白质固定化的底物。这项拟议的研究将试图辨别流体脂双层的低非特异性吸附特性是否在脂双层的交联和共价连接到底物时被保留。由此得到的组装体应该产生稳定的蛋白质-脂类组装体，其吸附的蛋白质膜具有定向呈现，并最大限度地保留蛋白质的结构和功能。

The goal of the proposed research is to prepare and characterize novel polymeric phospholipid bilayer assemblies as substrates for protein attachment. Immobilization of protein receptors on the bilayer surface has important practical applications for the development of new biosensors utilizing the bound protein as the biologically selective recognition element. The implementation of lipid bilayers as substrates for protein immobilization and subsequent use as biosensors requires that the chemical and structural properties be improved while maintaining the low nonspecific binding properties of lipid bilayer. The strategy to be investigated here is the lateral polymerization of the phospholipid bilayer in conjunction with site directed immobilization of proteins on the surface. The proposed research will involve preparation and characterization of novel tethered planar polymer phospholipid bilayers. The stability of the prepared cross- linked polymer phospholipid bilayers will then be assessed by solubility and optical measurements. The polymer lipid bilayers will subsequently be utilized as substrates for site directed protein immobilization. The proposed research will attempt to discern if the low nonspecific adsorption characteristic of fluid lipid bilayer are preserved upon cross-linking of the lipid bilayer and covalent attachment to the substrate. The resulting assembly should result in a stable protein- lipid assembly with an oriented presentation of the adsorbed protein film with maximum retention of protein structure and function.