GROWTH FACTORS AND LIGHT HISTORY IN PHOTORECEPTOR RESCUE

广泛的、长期的目标是了解视网膜如何适应 自身对环境光的变化保护感光细胞 防止退化并阐明这种适应的机制 系统被触发，并最终操纵这个系统来 增加感光细胞对退化的抵抗力。焦点 这项研究的建议是对基因和蛋白质进行表征 作为视网膜适应对BREAT反应的函数的表达 循环光，并了解这种适应如何保护 光感受器的退化。为了实现这一目标，基因和 碱性成纤维细胞生长因子、碱性成纤维细胞生长因子、成纤维细胞生长因子受体-1、CNTF、CNTF受体的蛋白表达 α、脑源性神经营养因子、trk B、IGF-1和IGF-1受体以及原癌基因 BCL-2的定量表征将通过执行Northern和 蛋白质印迹分析。随后，原位杂交和 将进行免疫细胞化学以定位表达。在……里面 此外，BREAT在成年大鼠视网膜中触发了这种适应系统 保护光感受器免受退化的循环光也将 检查过了。

The broad, long-term objective is to understand how retina can adapt itself to environmental light changes to protect photoreceptor cells from degeneration and to elucidate the mechanism whereby this adaptation system is triggered, and eventually, to manipulate this system to increase photoreceptor cell resistance to the degeneration. The focus of this research proposal is to characterize the genes and protein expression as a function of retinal adaptation in response to bright cyclic light, and to understand how this adaptation protects photoreceptors from degeneration. To achieve this goal, the gene and protein expressions of bFGF, aFGF, FGF receptor-1, CNTF, CNTF receptor- alpha, BDNF, trk B, IGF-1 and IGF-1 receptor, as well as proto-oncogene bcl-2 will be quantitatively characterized by performing Northern and Western blot analysis. Afterward, in situ hybridization and immunocytochemistry will be performed to localize the expression. In addition, such adaptation system in adult rat retina triggered by bright cyclic light to protect photoreceptors from degeneration will also be examined.