ENZYME THERMISTORS AS GLUCOSE SENSORS

描述（改编自申请人摘要）：本提案旨在 酶膜热敏电阻用作葡萄糖的可行性研究 I型糖尿病的发病机制申请人及其团体曾于 我在开发这种传感器方面做了大量的工作 尽管是为了不同的目的，他们发现热敏电阻有一个数字， 与所提出的电极型传感器相比具有许多优点。 总体目标是共价结合葡萄糖 氧化酶和过氧化氢酶结合到合适涂层的微薄层上，所述涂层 已应用于热敏电阻的玻璃体。几种可能的共同- 目前正在研究聚合物作为涂层的稳定性。 葡萄糖氧化酶分离自P. notatum，据报道， 一个非常低的公里氧气，将被用来试图克服"氧气 使用来自A.尼日尔.后 将酶成功地固定在热敏电阻上， 将在体外研究传感器的物理性质。然后 将在体内进行酶抑制剂的稳定性研究， 家兔，皮下和静脉注射，以测量对 血糖水平的突然变化。电子仪器和 处理来自晶闸管的信号所需的计算机程序具有 已经在美国国立卫生研究院设计了一个原型 仪器在P.I.的实验室将用于该项目。

DESCRIPTION (Adapted from applicant's abstract): This proposal aims to do a feasibility study on the use of enzyme coated thermistors as glucose sensors to in type I diabetes. The applicant and his group have previously done a considerable amount of work on the development of such sensors albeit for a different purpose and they find the thermistor has a number of advantages over the electrode-type sensors presenting being promoted. The overall aim is to covalently immobilize a combination of glucose oxidase and catalase onto a micro-thin layer of a suitable coating that has been applied to the glass body of the thermistor. Several possible co- polymers are presently being investigated for stability as a coating. Glucose oxidase isolated from P. notatum, which has been reported to have a very low Km for Oxygen, will be use to try to overcome the "oxygen deficit" problems encountered with use of the enzyme from A. niger. After the enzyme is successfully immobilized on the thermistor, the kinetic and physical properties of the sensors will be investigated in vitro. Then a stability study of the enzyme-thermistors in vivo will be conducted in rabbits, both subcutaneously and intravenously to measure response time to sudden changes in blood glucose levels. The electronic instrument and the computer program needed to process the signals from the thermistors have already been designed at the National Institutes of Health, an a prototype instrument in the P.I.'s laboratory will be used in this projects.