DEVELOPMENT OF AN ENTEROPATHOGENIC ESCHERICHIA COLI VACCINE

本项目的目的是鉴定致龋基因， 致病性大肠杆菌大肠杆菌（EPEC）的特异性基因敲除 在人类志愿者中的毒力突变体。的原位表达 假定的毒力基因也将通过分析mRNA进行测试， EPEC感染者空肠的内镜引导活检 志愿者特别是，这项研究将集中在斗争形成 菌毛（BFP），我们假设是必要的有效的细胞器， 肠道定植BFP基因簇的敲除突变 已通过等位基因置换制备，并将这些进行比较， 在人类志愿者中与亲本野生型菌株的毒力。如果BFP 被证实是一个毒力决定因子， 将制备含有BFP抗原的疫苗，并在人体中进行检测 志愿者

The purpose of this project is to identify genes of the diarrheagenic bacteria enteropathogenic E. coli (EPEC) by testing specific knockout mutants for virulence in human volunteers. In situ expression of putative virulence genes will also be tested by analyzing mRNA from endoscopically-directed biopsies of the jejenum from EPEC-infected volunteers. In particular, this study will focus on the bundle-forming pilus (BFP), an organelle that we hypothesize is necessary for efficient intestinal colonization. Knockout mutations of the BFP gene cluster have been prepared by allelic replacement and these will be compared for virulence in human volunteers with the parent wild-type strain. If BFP is confirmed to be a virulence determinant, vaccine candidates containing the BFP antigen will be prepared and tested in human volunteers.