CIRCULATING NITRIC OXIDE CONCENTRATION IN AGING RHESUS MACAQUES

目的探讨一氧化氮在恒河猴衰老过程中的可能作用。 猕猴模型。结果NO浓度呈下降趋势 在年长的绝经后猴子身上发现，而循环中的水平 NO似乎不受年龄或长期饮食的影响 对雄性恒河猴的限制。讨论一氧化氮(NO)有 建议在衰老过程中发挥作用，并相对 更年期低血压。饮食限制(DR)延缓衰老和 延长啮齿类动物的寿命，最近的研究表明 对恒河猴和食蟹猴健康的有益影响。在……里面 为了探讨NO在非人类衰老过程中的可能作用 在灵长类动物模型中，我们测量了循环中NO的水平 绝经前(n=9；年龄12.610.3岁)和绝经后(n=9) 雌性恒河猴10只，年龄26.810.4岁，青年恒河猴9只； 雄性恒河猴(11.910.5岁)和老年(n=10；26.711.0岁) 在雄性恒河猴(16-22岁)中， 接受30%DR 8年(n=12)和其匹配的对照组 自由进食(n=12)。绝经前的猴子略高一些 绝经后猕猴血清NO浓度为37.8912.79 5mvs. 31.2312.665 m，p=0.099。相比之下，年轻成年男性 猕猴的血清NO浓度与年长的雄性猕猴没有区别 37.8414.39 5m和37.1714.84 5m。此外，血浆中NO的值 在对照组和受限制雄性猴子之间没有差异34.0814.17 5m比33.6512.88 5m。NO略有下降的趋势 更年期猴子的血药浓度值得进一步研究。 调查。另一方面，循环中的NO水平不会 雄性恒河猴似乎受年龄或长期DR的影响 猴子。这些结果并不排除 局部的，即组织特异性的，对无数的 更年期过渡期间发生的生理变化和 作为博士未来方向的结果，我们计划进一步 探讨慢性阻塞性肺疾病患者循环中一氧化氮的关系及意义 与更年期转变和饮食限制的关系。钥匙 限制饮食，老龄化，更年期资助NIH PO1 AG11915

OBJECTIVE To explore the possible role of NO in aging in a rhesus macaque model. RESULTS A trend for somewhat lower NO concentrations was found in older, postmenopausal monkeys, while circulating levels of NO did not seem to be influenced by age or long-term dietary restriction in male rhesus monkeys. DISCUSSION Nitric oxide (NO) has been proposed to play a role in the aging process and to be relatively low during menopause. Dietary restriction (DR) retards aging and increases lifespan in rodents, and it has recently been shown to have beneficial effects on health in rhesus and cynomolgus macaques. In order to explore the possible role of NO in aging in this nonhuman primate model, we measured circulating levels of NO in group of premenopausal (n=9; aged 12.610.3 years [mean1SEM]) and postmenopausal (n=10; aged 26.810.4 years) female rhesus monkeys, young adult (n=9; aged 11.910.5 years) and old (n=10; aged 26.711.0 years) male rhesus monkeys, and in male rhesus monkeys (16-22 years old) that had undergone 30% DR for 8 years (n=12) and their matched control group fed ad libitum (n=12). Premenopausal monkeys had marginally higher serum NO concentration than postmenopausal monkeys 37.8912.79 5M vs. 31.2312.66 5M, respectively, p=0.099. In contrast, young adult male macaques did not differ from older males in serum NO concentration 37.8414.39 5M vs. 37.1714.84 5M, respectively. Also, plasma NO values did not differ between control and restricted male monkeys 34.0814.17 5M vs. 33.6512.88 5M, respectively. The trend for somewhat lower NO concentrations in the older, postmenopausal monkeys merits further investigation. On the other hand, circulating levels of NO do not seem to be influenced by age or by long-term DR in male rhesus monkeys. These results do not preclude an important role for localized, i.e., tissue specific, NO influences on the myriad physiological changes that occur during the menopausal transition and as a consequence of DR. FUTURE DIRECTIONS We plan to further investigate the relationship and significance of circulating NO in relation to the menopausal transition and dietary restriction. KEY WORDS Dietary restriction, aging, menopause FUNDING NIH PO1 AG11915