EFFECTS OF INFECTION OR VACCINATION ON INTRAEPITHELIAL LYMPHOCYTE POPULATIONS

目的采集肠道或生殖器粘膜组织标本 从恒河猴免疫前后或病毒感染。 将样品加工成mRNA和基于聚合酶链式反应的检测 用来量化单个T细胞受体基因的水平 在这些样本中表达。信使核糖核酸群体的进一步分析 是通过光谱分析完成的，其中单个T细胞 受体表达家族的区分是基于 表达基因的大小分布。光谱分型分析提供了 关于每个种群的特异性的其他信息和 感染或免疫后发生的变化。逆转录病毒 猕猴的感染引起了快速而广泛的转变 表达VGamma9 T细胞受体基因的T细胞群体。 这些变化比免疫后观察到的要大， 虽然两者都与对照标本有显著差异。 对女性患者的残留子宫样本进行了类似的研究， 显示VGamma9 T细胞受体表达的分布是 与未感染的猕猴相似。我们正在拓展的未来方向 用于检查其他VDelta和VGamma序列的探头面板 我们正在改善研究Vbeta基因表达的条件。 此外，还需要进一步的工作来改进抽样和抽样 处理程序。所有这些努力都是为了获得 更好地了解肠道和肠道中存在的淋巴细胞群 生殖器粘膜。关键词艾滋病、粘膜、免疫学、T细胞受体 资助NIH R01 AI/RR42534

OBJECTIVE Gut or genital mucosa tissue specimens were collected from rhesus macaques before and after immunization or virus infection. Samples were processed to preparation of mRNA and PCR-based assays were used to quantify the levels of individual T cell receptor genes expressed in these samples. Further analysis of the mRNA population was accomplished by spectrotyping analysis, wherein individual T cell receptor expressing families are distinguished on the basis of the size distribution of expressed genes. Spectrotyping analysis provides additional information about the specificity for each population and the changes occurring after infection or immunization. Retrovirus infection of macaques caused a rapid and extensive shift in the population of T cells expressing the Vgamma9 T cell receptor gene. These changes were larger than what was observed after immunization, though both were significantly different from control specimens. Similar studies done on residual uterine samples from women patients, showed a distribution of Vgamma9 T cell receptor expression that was similar to uninfected macaques. FUTURE DIRECTIONS We are expanding the panel of probes to examine additional Vdelta and Vgamma sequences and we are improving conditions for studying Vbeta gene expression. In addition, further work is necessary to improve sampling and sample handling procedures. All of these efforts are directed to gaining better understanding of lymphocyte populations present in the gut and genital mucosa. KEY WORDS AIDS, mucosa, immunology, T cell receptor FUNDING NIH R01 AI/RR42534