EFFICACY OF TRIPTOLIDE AS MALE CONTRACEPTIVE IN MARMOSET MONKEYS

摘要目的测定植物提取物的药效。 雷公藤甲素作为非人类灵长类动物的男性避孕药。 结果制定了生殖内分泌监测方案 (睾丸激素和黄体生成素)，行为(检测任何 活动模式或性欲的变化，随后可能会影响 生殖行为和/或生育力)和生殖生理 (由霍布森精子跟踪器量化的精子参数；体外和 活体生育力评估)具有最小的侵入性和社会性 扰乱绒猴受试者。新的绒猴生育协议 包括开发一种改进的精子采集方法，用于 绒猴振动器的刺激。通过修改多方案生育保健 个人振动器(依赖于振动器介导的阴茎传入 刺激，旨在收集脊髓损伤男性的精子； 也成功地在松鼠猴子身上使用)，精子样本 收集迅速(不到15秒)且可靠(80%-100% 成功率，取决于社会住房条件)来自6名男性 对其日常活动的干扰最小的绒猴。精子 可控震源刺激后的收集是一个重要的 对传统精液采集方法的改进 绒猴，例如电射精(30%成功率，需要 麻醉)和阴道冲洗(成功率45%-80%，需要很长时间 女性单人住房的周期和耗时的收集 会话)。利用我们新开发的产品的未来方向 确定雷公藤甲素作为男性的疗效的评估方案 雄性绒猴的避孕药。关键词男性避孕， 雷公藤甲素、精子、生育能力、精子采集、绒猴基金 康拉德MFG-97-27

OBJECTIVE To determine the efficacy of the plant extract Triptolide as a male contraceptive in a nonhuman primate species. RESULTS A protocol was devised to monitor reproductive endocrinology (testosterone and luteinizing hormone), behavior (to detect any changes in activity patterns or libido that may subsequently affect reproductive behavior and/or fertility), and reproductive physiology (sperm parameters quantified by a Hobsen Sperm Tracker; in vitro and in vivo fertility assessment) with minimal invasion and social disruption to marmoset subjects. The new marmoset fertility protocol included the development of an improved method of sperm collection for the marmoset vibrator stimulation. By modifying a Multicept FertiCare Personal Vibrator (which relies on vibrator-mediated penile afferent stimulation, designed to collect sperm from spinal cord injured men; also used successfully in squirrel monkeys), sperm samples were collected rapidly (less than 15 seconds) and reliably (80 - 100% success rate, depending on social housing conditions) from six male marmosets with minimal disturbance to their daily a ctivities. Sperm collection following vibrator stimulation was a significant improvement over the traditional sperm collection methods for marmosets, e.g. electroejaculation (30% success rate, requiring anesthesia) and vaginal washing (45-80% success rate, requiring long periods of single housing for females and time-consuming collection sessions). FUTURE DIRECTIONS To utilize our newly-developed assessment protocol to determine the efficacy of Triptolide as a male contraceptive in male marmosets. KEY WORDS male contraception, Triptolide, sperm, fertility, sperm collection, marmoset FUNDING CONRAD MFG-97-27