CORE--DNA

DNA Core将提供由各种项目确定的服务 PIS.这些措施将包括冷冻保存患者的淋巴细胞， 从血液中提取DNA，进行DNA测序和等位基因的聚合酶链式反应 序列测定和DNA合成为序列行走提供引物 和聚合酶链式反应。DNA核心将在解释 测序结果并将其提交给GenBank。核心将映射 由Project III和多个合作者提供的新cDNA，包括 李博士、马克森博士和索尔什博士。染色体的分配将通过聚合酶链式反应完成 到混合DNA面板。基因的定位将由FISH完成 其中来自人类Genethon MegaYAC文库的YAC对应于每个 候选基因的DNA。最后，核心将提供技术培训 和设备来支持Winograd博士，他将在现场进行 胎儿组织切片与cdna探针的杂交。苏立克博士 将在组织数据的解释方面进行合作。 这些活动将是现有遗传资源的延伸 提供DNA测序和合成的核心设施，淋巴母细胞 转化、基因定位(通过杂交面板DNA和FISH)、遗传 连锁分析(包括CEPH家系DNA)与一种生物试剂 医学院和大学的补给店。整合了 在现有结构内拟议的活动将确保 高效使用人员和资金。

The DNA Core will provide services as determined by the various project PIs. These will include cryopreservation of lymphocytes from patients, DNA isolation from blood, PCR reactions for DNA sequencing and allele determination and DNA synthesis to provide primers for sequence walking and PCR. The DNA core will provide assistance in the interpretation of sequence results and in their submission to GenBank. The Core will map new cDNAs provided by Project III and various collaborators including Drs. Lee, Maxson and Solursh. Chromosome assignment will be done by PCR to hybrid DNA panels. Localization of genes will be accomplished by FISH with YACs from the human Genethon MegaYAC library corresponding to each of the candidate cDNAs. Lastly, the Core will provide technical training and equipment to support Dr. Winograd, a fellow, who will conduct in situ hybridization of fetal histological sections with cDNA probes. Dr. Sulik will collaborate in the interpretation of the histological data. These activities will be an extension of the existing Genetic Resources Core Facility which provides DNA sequencing and synthesis, lymphoblast transformation, gene mapping (by hybrid panel DNAs and FISH), genetic linkage analysis (including CEPH pedigree DNAs) and a biological reagents supply store for the medical school and university. Incorporating the proposed activities within the present structure will assure the most efficient use of personnel and funds.