CORE--SHARED SERVICES CORE

在Patterson博士的指导下，共享服务核心与 福克斯女士和沃特斯女士的协助，将负责 行政便利和最大限度地提高研究生产率 该计划项目的。它将促进两国之间的互动 涉及的机构和调查人员以及外部国家 科学咨询委员会。它将有助于遵守所有 涉及使用人和动物的监管事项 拟开展的研究。共享服务核心将负责 体细胞杂交和亲本人类细胞的细胞遗传学分析 在杂交中，新的人类细胞(与临床和 分子核心)和啮齿动物(主要是小鼠和CHO)细胞用于 研究。核心将负责可行的、长期的存储 计划项目使用的大量细胞系集合 定期检测支原体污染的调查人员 计划项目要求的细胞生长和DNA制备 研究人员和生产新的体细胞杂交和 按要求建立新的原代细胞系。细胞遗传学 核心的职能将与组织培养部分密切合作 以便在任何时候进行适当的细胞遗传学分析 细胞解冻，或每当大规模培养时，例如用于DNA制备 或生化分析。核心将负责 获取所有细胞系及其细胞遗传学的计算机记录 特点。核心将继续负责发送 细胞和其他试剂给所有要求它们的调查人员。复本 最新的细胞遗传学分析和特殊说明，例如 关于生长条件，将例行发送。《戴维森》 分包是一项新的职能，将负责供应 计划项目调查人员使用T 65Dn(或其他)小鼠进行繁殖 目的：Northernblotts和胎鼠RNA样本，正常和 携带相应的16号染色体非整倍体，组织原位 用探针与小鼠不同组织(胎儿和成体)杂交 由计划项目调查员生成。超过当前授予的 期间，这个共享服务核心已经演变成一个极其高效的 它极大地协助了所有调查人员的工作，事实上， 世界各地的许多唐氏综合症研究人员。

The Shared Services core, under the direction of Dr. Patterson, with the assistance of Ms. Fox and Ms. Waters, will be responsible for administrative facilitation and maximization of the research productivity of the Program Project. It will facilitate interaction between the Institution and investigators involved and the outside National Scientific Advisory Committee. It will aid with compliance with all regulatory matters involving the use of humans and animals in the proposed research. The Shared Services Core will be responsible for cytogenetic analysis of somatic cell hybrids, parental human cells used in hybrids, new human cells (in collaboration with the Clinical and Molecular Core), and rodent (primarily mouse and CHO) cells used in the research. The core will be responsible for viable, long term storage of the large collection of cell lines used by the Program Project investigators, for periodic testing for mycoplasm contamination, for growth of cells and DNA preparation as requested by Program Project investigators and for production of new somatic cell hybrids and establishment of new primary cell lines as requested. The cytogenetic function of the core will work closely with the tissue culture component so that appropriate cytogenetic analyses will be undertaken whenever cells are thawed, or whenever large cultures, e.g. for DNA preparation or biochemical analysis, are undertaken. The Core will be responsible for computerized records of all cell lines and their cytogenetic characteristics. the Core will continue to be responsible for sending cells and other reagents to all investigators requesting them. Copies of up-to-date cytogenetic analyses and special instructions, for example regarding growth conditions, will be sent routinely. The Davisson subcontract is a new function and will be responsible for supplying Program Project investigators with T 65Dn (or other) mice for breeding purposes, Northern blots and RNA samples of fetal mice, both normal and carrying relevant chromosome 16 aneuploidies, and for tissue in situ hybridization to various mouse (fetal and adult) tissues using probes generated by Program Project investigators. Over the current granting period, this Shared Services Core has evolved into an extremely efficient unit which greatly assists the work of all the investigators, and indeed, many Down Syndrome researchers worldwide.