X RAY DIFFRACTION EQUIPMENT FOR MACROMOLECULES

该提案的目标是为第二个项目提供部分资金， 分子生物物理部用X射线面积探测器的 Hauptman-Woodward医学研究所，以及 Rigaku R-Axis II的翻新和改造。这 设备将由六个主要用户组共享， 正在进行由PHS资助的研究的主要研究者 以及一些其他方式资助的项目。所需资金 将用于购买Rigatu ~UltraX”旋转阳极发生器， ADSC四模块CCD探测器系统、聚焦镜、K 一个专用的SGI， 工作站第二个探测器的存在将允许 对现有的R-Axis II进行了修改，配备了 石墨单色器，无法常规目标的变化。的 增加了单色枢轴和三轴读数， 获取Cr目标Win允许目标改变，同时最小化 停机时间。然后，该仪器可用于正常数据收集 (Cu)，并利用异常散射技术直接 方法，通过使用最长的 实际波长（Cr）。此外，图像板，激光， ”“是，是。 预期寿命，将被替换。内部咨询委员会 由研究所所长、执行副所长、 研究，和分子生物物理系主任。将有 全面负责设备和日常工作， 管理工作将由科学家提供， 监督研究所的共享衍射设施， 该部门的另外两名科学工作人员。的项目 包括类固醇的研究 代谢酶，类固醇转运蛋白，离子转运 抗生素，二氢叶酸还原酶/抑制剂复合物， 甲状腺素运载蛋白复合物、雌激素芳香化酶及其与 单克隆抗体，叶酰聚谷氨酸合成酶，木聚糖酯酶， 和牛胰蛋白酶的研究，以及新的相分离方法的研究 保持战略定力购置这一设备的总费用 和现有设备的改造将减少 已从私人来源获得的230 000美元， 扩展蛋白质衍射设备。

The goal of this proposal is to provide partial funding for a second X-ray area detector for use in the Molecular Biophysics Dept. of the Hauptman-Woodward Medical Research Institute, and for the refurbishing and modification of the existing Rigaku R-Axis II. This equipment is to be shared by a major user group consisting of six principal investigators who are conducting research funded by the PHS and a number of projects funded by other means. The requested funds will be used to purchase a Rigatu ~UltraX" rotating anode generator, an ADSC four module CCD detector system, focusing mirrors, K goniostat and low temperature device, and a dedicated SGI workstation. The presence of a second detector will permit the modification of the existing R-Axis II, which is equipped with a graphite monochromator, to unable routine target changes. The addition of the monochromator pivot and three-axis readout and the acquisition of a Cr target win allow target changes while minimizing down time. The instrument can then be used for normal data collection (Cu), and to exploit anomalous scattering techniques in direct methods, maximizing the anomalous signal by using the longest wavelength practical (Cr). Additionally, the image plates, laser, bearings, belts and Be window, which are nearing the end of their expected life, will be replaced. An internal advisory committee consisting of the Institute's President, Executive Vice-President-- Research, and the Head of the Molecular Biophysics Dept. will have overall responsibility for the equipment, and the day-to-day management will be provided by the staff scientist who presently supervises the Institute's shared diffraction facilities, assisted by two other members of the department's scientific staff. The projects to be served by this equipment include the study of steroid metabolizing enzymes, steroid transport proteins, ion transport antibiotics, dihydrofolate reductase/inhibitor complexes, transthyretin complexes, estrogen aromatase and its complex with a monoclonal antibody, folylpolyglutamate synthetase, xylan esterases, and bovine duodenase, as well as the research on new methods of phase determination. The total cost of the acquisition of this equipment and modification of existing equipment will be reduced by an amount of $230,000 already obtained from private sources for the purpose of expanding the protein diffraction facilities.