CORE--MONOCLONAL ANTIBODY

这个核心将为几个项目提供单克隆抗体， 维持和储存产生单克隆抗体的细胞系，并提供 为SCOR研究人员提供抗体纯化专业知识。 如果在稍后 项目的阶段，抗体的“人源化”是必需的，核心是 具有抗体可变区基因测序技术， 用于构建嵌合抗体的分泌细胞，以及 人源化含有人重链和 允许连接的轻链恒定区基因和限制性位点 转染到鼠骨髓瘤细胞中的鼠源VH或VL 线 将为项目1、3、4、 5和6。 针对项目4中的CFTR-FLAG构建体制备的抗体 也可以用于项目2。

This core will provide monoclonal antibodies for several of the projects, maintain and store monoclonal antibody producing cell lines, and provide antibody purification expertise for SCOR investigators. If at a later stage of the projects, "humanization" of antibodies is required, the core has the technology to sequence the variable domain genes of antibody secreting cells for use in construction of chimeric antibodies and humanization of murine monoclonal antibodies containing human heavy and light chain constant domain genes and restriction sites allowing joining of the VH or VL of murine origin transfected into murine myeloma cell lines. Antibodies will be prepared and provided for Projects 1, 3, 4, 5, and 6. Antibodies prepared to the CFTR-FLAG constructs in Project 4 may be used in Project 2 as well.