REGULATION OF CYTIDYLYLTRANSFERASE ACTIVITY

这个实验室的工作重点是对哺乳动物的调节 磷脂酰胆碱代谢，由酶调节 胆碱激酶和CTP：磷酸胆碱胞苷转移酶(CCT)。我们 假设细胞胆碱激酶活性受 胆碱激酶同工酶在某些细胞和细胞中的选择性表达 对某些信号的反应。我们正在孤立、表达和 人胆碱激酶亚型的表达模式分析 哺乳动物和线虫，线虫。此外，我们正在研究 磷酸化和脂类对CCT活性的调节，并已被 研究了该酶的催化机理。我们使用重组DNA 所有这些研究的技术，并需要使用GCG数据 碱基和序列分析软件来继续它们。

Work in this laboratory has focused on the regulation of mammalian phosphatidylcholine metabolism, which is regulated by the enzymes choline kinase and CTP:cholinephosphate cytidylyltransferase (CCT). We hypothesize that cellular choline kinase activity is modulated by the selective expression of choline kinase isozymes in certain cells and in response to certain signals. We are isolating, expressing, and analyzing the expression patterns of choline kinase isoforms from mammals and the nematode, C. elegans. In addition, we are studying the regulation of CCT activity by phosphorylation and lipids, and have been studying the catalytic mechanism of this enzyme. We use recombinant DNA techniques for all these studies, and require the use of the GCG data base and sequence analysis software to continue them.