COPPER HOMEOSTASIS IN YEAST

实验室研究的重点是对生物多样性的调查 大鼠局灶性脑缺血再灌注损伤中金属硫蛋白基因1的表达调控 酿酒酵母。金属硫蛋白是一种小半胱氨酸 在大多数真核生物中发现的丰富蛋白质，可以与重金属结合，以及 从而保护细胞免受毒害。CUP-1基因的表达 在存在铜或铜等金属离子时，酵母中的 银牌。这种诱导是通过位于上游的元素来调节的 在Cup1的启动子区域中，该区域充当与 交易因子，ACE1。在没有金属离子的情况下，ACE1存在 作为一种游离蛋白，CUP1的表达水平很低。当着大家的面 在铜离子中，ACE1与CUP1启动子结合并诱导 表情。ACE1基因在本实验室进行了鉴定和克隆。

The focus of the laboratory research is an investigation of the regulation of expression of the metallothionein locus, CUP 1, in the yeast Saccharomyces cerevisiae. Metallothioneins are small cysteine rich proteins found in most eukaryotes, which bind to heavy metals, and thus protect the cell from toxification. Expression of the CUP 1 gene in yeast is increased in the presence of metal ions such as copper or silver. This induction is mediated through upstream elements located in the promoter region of Cup1, which serve as binding sites for the transacting factor, ACE1. In the absence of metal ions, ACE1 exists as a free protein, and expression of CUP1 is very low. In the presence of copper ions, ACE1 binds to the CUP 1 promoter and induces expression. The ACE1 gene was identified and cloned in this laboratory.