HUMAN CEREBRAL GABA METABOLISM STUDIES IN VIVO WITH MR SPECTROSCOPY

我们建议联合使用黄体生成素和~(13)C核磁共振研究癫痫患者的脑谷氨酸和GABA代谢。对两种新型抗癫痫药物的反应将作为探针，以阐明对GABA水平升高的代谢反应。我们继续研究体内合成GABA的速率、谷氨酸和脱羧酶(GAD)活性以及GABA浓度之间的关系。应用连续单光子发射计算机断层扫描(SPECT)和核磁共振氢谱(1H NMRS)技术，评价人脑GABA升高对作为GABA-A受体标志物的苯二氮平受体表达的影响。通过连续测量服用加巴喷丁前后大脑谷氨酸、GABA和谷氨酰胺周转率，探讨服用加巴喷丁后人脑GABA增加的机制。

We propose to study brain glutamate and GABA turnover inpatients with epilepsy using combined lH and 13C NMRS. The responses to two novel anti-epileptic drugs will serve as probes to clarify the metabolic response to increased levels of GABA. We continue to investigate the relationship between in vivo rate of GABA synthesis, glutamic acid and decarboxylase (GAD) activity, and GABA concentration. Assess the effects of increased Human Brain GABA on the expression of Benodiazapine receptors as a marker for GABA-A receptors using serial single photon emission computer tomography (SPECT) and 1H Magnetic Resonance Spectroscopy (1H NMRS). Explore the mechanisms associated with the increase in Human Brain GABA following Gabapentin administration using serial measurements of the rate of cerebral glutamate, GABA, and glutamine turnover before and after the administration of Gabapentin.