Core--Molecular and cellular imaging

分子和细胞成像核心（MCIC）将是一个本地和 用于基于组织的图像分析的区域资源。MCIC将提供 用于定性和定量胶片密度测定的最先进设备 和显微组织分析， research. MCIC将为感兴趣的用户提供培训或执行 对核准项目的选定分析。预计核准的项目 来自当地和区域调查员的信息MCIC使用的申请将 另外还征求了与衰老有关的科学家的无偏见体视学， 平铺映射和三维重建。基于组织的技术包括 定量受体放射自显影和原位组织化学 将在审查的基础上对老龄化相关项目进行评估。这些 各种类型的分析和技术需要专业知识， 可用于许多涉及老化研究的实验室。 将开发新的原位杂交探针和方案， 经验证，并提供给当地、区域和国家调查人员。 与微阵列分析核心（MAC）和基因 表达载体核心（GEVC），我们将开发，验证，并提供 5个针对ISHH的细胞管家基因的探针， 验证从微阵列获得的数据。寡核苷酸或cRNA 针对地方、区域或国家感兴趣的特定基因的探针 研究人员将被设计或克隆，对人体组织感兴趣， 年龄相关疾病的动物模型。这些新基因将被 通过微阵列分析或传统方法鉴定新基因 识别. 作为开发，我们的网站将包含一个服务和探针列表 提供给潜在用户和所有图像分析协议的副本 和ISSH。

The Molecular and Cellular Imaging Core (MCIC) will be a local and regional resource for tissue-based image analysis. The MCIC will provide state-of-the-art facilities for qualitative and quantitative film densitometry and microscopic tissue analyses for investigators in the field of aging research. The MCIC will provide training to interested users or perform selected analyses of approved projects. Approved projects are anticipated from local and regional investigators. Applications for MCIC use will additionally solicited form aging-related scientists unbiased stereology, tiled mapping, and 3-D reconstruction. Tissue based techniques including quantitative receptor autoradiography and in situ histochemistry (ISHH) will be performed on a reviewed basis for aging-related projects. These types of analyses and techniques require expertise that may not be available to many laboratories involving in aging studies. Novel in situ hybridization probes and protocols will be developed, validated, and made available to local, regional and national investigators. In collaboration with the Microarray Analysis Core (MAC) and the Gene Expression Vector Core (GEVC) we will develop, validate, and provide five probes to cellular housekeeping genes for ISHH that can be used for validation of data obtained from microarrays. Oligonucleotide or cRNA probes to specific genes of interest to local, regional, or national researchers will be designed or cloned, to be of interest in human tissue or animal models of age-related diseases. These novel genes will be identified by microarray analyses or traditional methods of novel gene identification. As developed, our web site will contain a list of services and probes available to potential users and copies of all protocols for image analyses and ISSH.