CORE--VIRUS/PRIMARY CELL FACILITY

病毒-初级细胞核心的目的是为病毒和初级细胞研究提供全面支持，以提高宾夕法尼亚大学/维斯塔尔大学/CHOP社区内现有艾滋病毒调查人员的生产力和效率，便利新的调查人员进入艾滋病研究领域，并促进这些调查人员之间的科学合作。该核心设施将提供：(1)一个受仔细监督的设施，用于生物安全3级培训，并供调查人员在不接触个别封闭设施或需要比目前可用的更高水平的封闭水平的情况下进行封闭实验；(2)一个中央资料库，存储代表致病机制不同方面的原型艾滋病毒-1毒株、突变病毒株和特征良好的初级病毒分离株；(3)提供艾滋病毒-1初级分离、滴定和鉴定服务；以及(4)一个专门的原代细胞方案，它将促进利用与艾滋病毒研究有关的专门原代细胞类型(通过为试点实验提供细胞，培训专门细胞类型的分离研究，并促进协作互动)，并通过向血液单个核细胞提供对艾滋病毒研究重要的已确定的基因类型(CCR5、CCR2b、SDF-1)。我们期望，这一核心将加强现有艾滋病毒调查员之间的合作和生产力，并便利非艾滋病毒调查员进入实地，同时提供加强实验室生物安全的机制。

The purpose of the Virus-Primary Cell Core is to provide comprehensive support for virus and primary cell-based studies in order to enhance the productivity and efficiency of established HIV investigators within the Penn/Wistar/CHOP community, facilitate the entrance of new investigators into the field of AIDS research, and promote scientific collaborations among these investigators. The core will provide: (1) a carefully supervised facility for Biosafety Level 3 training and for the performance of containment experiments by investigators without access to individual containment facilities or who require a level of containment greater than currently available; (2) a central repository of prototype HIV-1 strains, mutant virus strains, and well-characterized primary virus isolates representing different aspects of pathogenesis; (3) a service for primary HIV-1 isolation, titration and characterization, and; (4) a specialized primary cell program that will facilitate utilization of specialized primary cell types relevant to HIV studies (by providing cells for pilot experiments, training of investigations in the isolation of specialized cell types, and facilitating collaborative interaction) and by providing blood mononuclear cells with defined genotypes important for HIV studies (CCR5, CCR2b, SDF-1). It is our expectation that this core will enhance collaborations and productivity among existing HIV investigators and facilitate the entrance of non-HIV investigators into the field while providing a mechanisms to enhance laboratory biosafety.