CORE--STRUCTURAL BIOLOGY FACILITY

结构生物学核心将是一个共享的中心资源，使宾夕法尼亚大学的研究人员能够为他们关于艾滋病相关大分子的研究项目带来分子和结构分析和理解。分子机制、分子结构和药物设计都是该大学目前资助的研究课题。它们也是即将到来的几个项目中的常见主题。这些项目以及与艾滋病相关的大分子生物学项目的一个共同需要是能够使用结构生物学方法，以便更多地了解结构和机制，并将这些信息用于基于结构和机制的拮抗剂设计。结构生物学核心的目标将是提供服务，促进利用结构生物学解决与艾滋病有关的研究问题。结构生物学核心的四个主要功能将是：(1)蛋白质生产，包括扩大表达和纯化，为结构和机制研究提供材料；(2)相互作用分析，侧重于大分子相互作用动力学的光学生物传感器分析，以及对艾滋病毒-1病毒和艾滋病发病机制组装机制的表征；(3)结构-功能分析，包括高分辨率结构的分子图形可视化和突变分析的结构指导；(4)资源联网，特别是指导用户使用核心之外的技术资源，进行专业的生物物理、计算和高分辨率结构确定。宾夕法尼亚大学CFAR的一个关键优势将是将研究免疫缺陷病毒和艾滋病的生物学和化学的研究人员聚集在一起，利用资源阐明分子和结构特性，并将这些特性用作药物设计的主要信息。将鼓励生物学家、化学家和结构生物学家之间的协调研究和合作。结构生物学核心将提供一个集中的资源，提供蛋白质材料，启动相互作用分析和结构-功能研究，促进和领导与艾滋病有关的项目进入结构生物学领域，从而帮助实现宾夕法尼亚大学艾滋病研究的分子、结构和药物设计目标。

The Structural Biology Core will be a shared central resource to enable researchers at the University of Pennsylvania to bring molecular and structural analysis and understanding to their research projects on AIDS- related macromolecules. Molecular mechanism, molecular structure and drug design all are subjects of currently funded research at the University. They also are common themes in several upcoming projects. A common need in these projects as well as those on the biology of AIDS-related macromolecules is to enable the use of structural biology approaches in order to learn more about structure and mechanism and to use that information for structure- and mechanism-based antagonist design. The goal of the Structural Biology Core will be to provide services which facilitate the use of structural biology to solve AIDS-related research problems. The four main functions of the Structural Biology Core will be: (1) Protein Production, including scaled up expression and purification to provide materials for structural and mechanistic studies; (2) Interaction Analysis, focusing on optical biosensor analysis of macromolecular interaction kinetics and characterization of assembly mechanisms in HIV-1 and the pathogenesis of AIDS; (3) Structure-Function Analysis, including molecular graphics visualization of high resolution structures and structural guidance for mutagenic analysis; and (4) Resource Networking, in particular guiding users to technology resources beyond the Core for specialized biophysical, computational and high resolution structure determination. A key strength of the CFAR at the University of Pennsylvania will be to bring together researchers investigating the biology and chemistry of immunodeficiency viruses and AIDS with the resources to elucidate molecular and structural properties and to use these properties as lead information in drug design. Coordinated research and collaborations will be encouraged amongst biologists, chemists and structural biologists. The Structure Biology Core will provide a centralized resource which will provide protein materials, initiate interaction analyses and structure-function studies, facilitate and lead AIDS-related projects into the structural biology area and hence help meet the molecular, structural and drug design goals of AIDS research at UPenn.