Tissue-Specific Antioxidant Antimutagenesis

描述：（由申请人提供）抗氧化剂对 近年来已经广泛认识到衰老和变性疾病， 每年花费数十亿美元用于大剂量抗氧化剂补充剂。的 现代自由基衰老理论假定， 抗氧化剂/促氧化剂平衡，导致氧化应激增加， 细胞功能失调和衰老。在这一理论的背景下， 抗氧化剂可以影响主要的“内在”老化过程， 几个继发性年龄相关的病理过程。几 流行病学和临床研究揭示了饮食的潜在作用， 抗氧化剂在与年龄相关的自身免疫性疾病， 癌症的发病率和死亡率。大蓝转基因小鼠突变 检测系统允许氧化应激，诱变， 和致癌作用进行研究的方式以前不可能。大 蓝色系统将用于测量膳食抗氧化剂对 突变频率和模式，以及正常条件下的肿瘤发生 和升高的内源性氧化应激。这些结果可能会让我们深入了解 组织特异性氧化应激的性质和程度， 为未来研究改变发病率或进展奠定基础 肿瘤发生这些结果将对我们理解 人类自发体细胞突变，并确定最大的 分别补充维生素E、C和β-胡萝卜素的抗突变作用 或者在鸡尾酒里

DESCRIPTION: (provided by applicant) The potential impact of antioxidants on aging and degenerative diseases has been widely recognized in recent years and billions of dollars are spent annually on megadose antioxidant supplements. The modem free radical theory of aging postulates a shift in the antioxidant/pro-oxidant balance that leads to increased oxidative stress, dysregulation of cellular function, and aging. In the context of this theory, antioxidants can influence the primary "intrinsic" aging process as well as several secondary age-associated pathological processes. Several epidemiological and clinical studies have revealed potential roles for dietary antioxidants in age-associated autoimmune diseases, and the reduction of risk of morbidity and mortality from cancer. The Big Blue transgenic mouse mutation detection system allows the relationship between oxidative stress, mutagenesis, and carcinogenesis to be examined in a manner not previously possible. The Big Blue system will be utilized to measure the effects of dietary antioxidants on mutation frequency and pattern, and tumorigenesis under conditions of normal and elevated endogenous oxidative stress. The results may provide insight into the nature and extent of tissue-specific oxidative stress and lay the foundation for future studies to modify the incidence or progression of tumorigenesis. The results will have implications for our understanding spontaneous somatic mutation in humans and for defining the maximal antimutagenic effect of vitamin E, C and beta-carotene supplements separately or in cocktails.