BIOSYNTHESIS OF PARAMYXOVIRUSES

描述：拟议的研究将集中在副流感病毒 通过研究病毒与病毒之间的蛋白质-蛋白质相互作用 核衣壳、基质蛋白和病毒包膜糖蛋白。 要求1 将决定蛋白质之间的相互作用， 血凝素-神经氨酸酶（HN）掺入感染性病毒 颗粒，并将使用定点诱变，以确定关键的氨基酸 HN细胞质尾区的酸性残基， 在一些实施方案中，HN可以通过核衣壳结合到病毒包膜中以允许HN掺入病毒包膜中。 目的2 将决定核衣壳掺入病毒的机制 粒子 仙台和人副流感病毒组成的核衣壳 1型嵌合NP分子将被表达， 对于组装到病毒包膜中至关重要的NP残基将 被阐明。 目标3提出研究结构/功能关系 副流感病毒的基质蛋白。 仙台病毒 温度敏感（TS）基质突变体可以用 cDNA表达的野生型M蛋白。 突变（互补） 蛋白质可以允许功能结构域的定义。

DESCRIPTION: The proposed research will focus on parainfluenza virus assembly by studying protein-protein interactions between the viral nucleocapsid, matrix protein, and the viral envelope glycoproteins. Aim 1 will determine the protein-protein interactions that are responsible for the incorporation of the hemagglutinin-neuraminidase (HN) into infectious virus particles, and will use site-directed mutagenesis to identify critical amino acid residues of the cytoplasmic tail of HN that interact with the nucleocapsid to allow incorporation of HN into the viral envelope. Aim 2 will determine the mechanism of nucleocapsid incorporation into virus particles. Nucleocapsids composed of Sendai and human parainfluenza virus type 1 chimeric NP molecules will be expressed and the domains and specific residues of NP that are critical for assembly into the virus envelope will be elucidated. Aim 3 proposes to study the structure/function relationship of the matrix protein of parainfluenza viruses. Sendai virus temperature-sensitive (ts) matrix mutants can be complemented with a cDNA-expressed wild type M protein. Mutagenesis of the (complementing) protein may allow the defining of functional domains.