CORE--BONE HISTOMORPHOMETRY

这一组织形态计量学核心一直是我们SCOR计划的组成部分 自1987年成立以来。在本申请中，核心提供 为五个项目中的四个项目提供组织形态计量支助 三维结构评估的可行性方案。在整个过程中 五年内，核心将准备和分析600多只大鼠和小鼠 标本和200份人体活组织标本。将采用骨组织形态计量学 鉴定缺乏骨钙素的转基因小鼠的骨骼表型 P53抑癌基因。骨组织形态计量学将得到广泛应用 探讨甲状旁腺素的合成代谢作用及其相互作用机制 膳食缺钙与PT给药之间的关系。骨 组织形态计量学将被用来表征细胞和 甲状旁腺切除术对绝经后轻度甲状旁腺功能的影响 原发性甲状旁腺功能亢进症。骨组织形态计量学将用于探索 甲状旁腺激素最初强烈合成代谢作用的机制 患有骨质疏松症。通过Micro-MRI评估的结构变量将是 松质骨和松质骨的组织形态计量学变量相关 皮质骨结构。这个核心将采用最先进的技术 并将为样品的分析带来超过15年的经验 以及对数据的解释。

This histomorphometry core has been in integral part of our SCOR program since its inception in 1987. In the present application, the core provides histomorphometric support for four of the five projects and for the feasibility project on assessment of 3-d structure. Over the course of the five years the core will prepare and analyze over 600 rat and mouse specimens and 200 human biopsies. Bone histomorphometry will be employed to characterize the skeletal phenotype of transgenic mice which lack the p53 tumor suppressor gene. Bone histomorphometry will be used extensively to explore the mechanism of the anabolic action of PTH and interaction between dietary calcium deficiency and PT administration. Bone histomorphometry will be employed to characterize the cellular and structural effects of parathyroidectomy in postmenopausal women with mild primary hyperparathyroidism. Bone histomorphometry will be used to explore the mechanism of the initial intense anabolic action of PTH in patients with osteoporosis. Structural variables, assessed by Micro-MRI, will be correlated with histomorphometric variables of both cancellous and cortical bone structure. This core will employ state of the art technology and will bring over 15 years of experience to the analysis of the samples and interpretation of the data.