CORE--CELL CULTURE MEDIA PREPARATION FACILITY

细胞培养基制备设施（位于实验室721室） McArdle实验室）由Janet Mertz博士指导。 这个220平方英尺的设施配备了层流食品， 水蒸馏装置，多个过滤装置，天平， 一个pH计，一个37摄氏度的培养箱，和几个冰箱。女士 Kathy约翰逊根据 每个调查员的需求。去年她准备了3,800多升 这些培养基包括高糖DMEM、Joklik、S-MEM、DMEM 不含酚红RPMI-1640和F-12约翰逊女士还准备了特别的 缓冲液和培养基如缺乏特定氨基酸的DMEM。她保证 每批培养基的无菌性，通过检测其生长情况， 污染物，并保持它3至4周，直到所有测试证明 阴性在过去的一年里，约翰逊女士还准备了细菌 和酵母工作约8,150板含有各种营养素， 药物和指标。这些培养基储存在1/2和1升瓶中 在721房间的冰箱里整个麦卡德尔的研究人员 实验室根据需要检查这些介质，并注明数量 这样，配料的成本就可以被收回到 个别研究小组。

The Cell Culture Media Preparation Facility (located in room 721 of the McArdle Laboratory) is under the faculty supervision of Dr. Janet Mertz. This 220 square foot facility is equipped with a laminar flow food, a water distillation apparatus, multiple filtration apparatuses, a balance, a pH meter, a 37 degree Celsius incubator, and several refrigerators. Ms. Kathy Johnson prepares and sterilizes cell culture media according to the needs of each investigator. Last year she prepared more than 3,800 liters of media; these media included DMEM with high glucose, Joklik, S-MEM, DMEM without phenol red, RPMI-1640 and F-12. Ms. Johnson also prepares special buffers and media such as DMEM lacking specific amino acids. She insures the sterility of each batch of medium by testing it for growth of contaminants and holding it for 3 to 4 weeks until all tests prove negative. During the last year, Ms. Johnson also prepared for bacterial and yeast work approximately 8,150 plates containing various nutrients, drugs, and indicators. These media are stored in 1/2 and 1 litter bottles in refrigerators located in room 721. Researchers throughout the McArdle Laboratory check out these media as needed, indicating the quantities taken so the costs of the ingredients can be charged back to the individual research groups.