CORE--FLUORESCENT IN SITU HYBRIDIZATION/CYTOGENETICS

此核心的主要目标是：1)比较 间期FISH、超中期FISH和RT-PCR 能够预测预后，监测治疗反应，以及 预测复发，以及2)支持所需的基因图谱研究 对于Lalitha Nagarajan博士来说，定义 7号染色体与7q-1预后不良有关 病人。 这个核心将对中间相核执行FISH以检测 染色体易位的白血病细胞，如t(15；17) 和t(9；22)，2)研制用于inv(16)和t(8；21)的界面探针 与西西里亚诺、克莱克斯顿和张博士合作，以及 与合作开发5q和7q的相间探头 Nagarajan博士将iFish结果与超中期FISH相关联 (HMF)和RT-PCR以支持基因所需的FISH研究 绘制7号染色体上的图谱，以确定该染色体上的关键区域 导致不良预后的染色体(拉利萨博士) Nagarajan)。 这个核心将与Elihu Estey博士合作监测治疗 回应，Richard Champlin博士监测对骨髓移植的反应，Dr。 克里斯托弗·雷丁丰富二倍体干细胞，关联iFish， HMF、RT-PCR和Lalitha Nagarajan博士绘制了关键的 7号染色体上导致预后不良的区域。

The major goals of this core are: 1) to compare the utilities of interphase FISH, hypermetaphase FISH, and RT-PCR in their abilities to predict prognosis, monitor therapy responses, and predict relapse, and 2) to support the gene mapping studies needed for Dr. Lalitha Nagarajan to define the critical regions on chromosome 7 that are responsible for poor prognosis of 7q- patients. This core will perform 1) FISH on interphase nuclei to detect leukemic cells with chromosomal translocations such as t(15;17) and t(9;22), 2) develop interphase probes for inv (16) and t(8;21) in collaboration with Drs. Siciliano, Claxton and Chang, and develop interphase probes for 5q- and 7q- in collaboration with Dr. Nagarajan to correlate iFISH results with hypermetaphase FISH (HMF) and RT-PCR to support the FISH studies needed for gene mapping on chromosome 7 to define the critical regions on this chromosome that are responsible for poor prognosis (Dr. Lalitha Nagarajan). This core will collaborate with Dr. Elihu Estey to monitor therapy responses, Dr. Richard Champlin to monitor responses to BMT, Dr. Christopher Reading to enrich diploid stem cells, correlate iFISH, HMF, and RT-PCR, and Dr. Lalitha Nagarajan to map the critical regions on chromosome 7 that are responsible for poor prognosis.