CORE--ANIMAL

颅面分子生物学中心将包括一个动物核心 支持中心研究人员的研究努力的设施。 这四个项目都需要使用实验动物才能完成 这项拟议的研究旨在研究颅面发育的机制。 处于明确的妊娠阶段的小鼠，特殊品系的小鼠和 转基因小鼠品系将被保存在间隔室设施中 由南加州大学隔间分校分配给 CCMB。动物核心将监督有关住房的方方面面， 项目中使用的实验动物的饲养和维护 由中心提供支持。将对畜牧业进行监测，以 确保温度、明暗循环、笼子、床铺材料、 食物、水和每个笼子里的动物数量符合标准 都是必需的，并且都是最佳的，以消除 实验程序，并提供可重现的组织来源 实验。小鼠的群体将在很好的控制下繁殖 有2小时交配期的情况和随后的阴道检查 塞子。对繁殖的控制是允许对 妊娠期特定时间点的组织与下列事件相关 四个项目中的每一个都在研究头面部发育。这个 动物核心将协助调查人员上演 胚胎和胎儿的发育(泰勒分期)，在 所需实验组织的显微解剖，在 颅面畸形和辨别期望的动物 遗传型或表型特征。动物核心将协调 动物使用，以便最大限度地利用来自每个实验的组织 动物就会出现。小鼠动物模型系统将被用作 正常发展的范例以及确定 先天性颅面部畸形的病因和分子发病机制 畸形。

The Center for Craniofacial Molecular Biology will include an Animal Core Facility to support the research efforts of the center investigators. The four projects all require the use of experimental animals to complete the proposed studies examining mechanisms of craniofacial development. Mice at well defined stages of gestation, special strains of mice and transgenic mouse lines will be maintained in the vivarium facilities allocated by the University of Southern California Vivaria for use by the CCMB. The Animal Core will oversee all aspects concerning the housing, breeding and maintenance of the experimental animals used in the projects supported by the Center. The animal husbandry will be monitored to insure that temperature, light-dark cycles, cages, bedding materials, food, and water and numbers of animals per cage conform to the standards required and are optimal both to eliminate confounding variables in the experimental procedures and to provide reproducible source of tissue for experimentation. The colonies of mice will be bred under well controlled conditions with a 2 hour mating period and subsequent check for vaginal plugs. The control of breeding is essential to permit examination of tissues at defined points in gestation associated with the events in craniofacial development being studied in each of the four projects. The Animal Core will provide assistance to the investigators in staging the development of the embryos and fetuses (Theiler staging), in microdissection of the desired experimental tissues, in documentation of craniofacial malformations and in identifying animals with desired genotypic or phenotypic characteristics. The Animal Core will coordinate animal use so that the greatest use of the tissues from each experimental animal will occur. The mouse animal model systems will be used as paradigms for normal development as well as for identification of the etiology and molecular pathogenesis of congenital craniofacial malformations.