CORE--HLA TYPING

该项目的核心分为两个部分。A部分是人类白细胞抗原 本研究中所有糖尿病患者的分型 提案--主要是在大学的芭芭拉·戴维斯糖尿病中心 科罗拉多大学的内分泌学诊所和华盛顿大学的内分泌学诊所 威斯康星医学院的儿科内分泌学诊所。在……里面 此外，少数患者将在斯坦福大学进行研究 来自露西尔儿科内分泌学诊所的大学 索尔特儿童医院。在这个核心中，亨利·埃利希博士将使用 基于聚合酶链式反应的人类白细胞抗原DR、B1和DQ基因分型方法 本研究拟研究患者的B1型。因为这三个人 大糖尿病诊所和一个小糖尿病诊所之间有250到300个新糖尿病患者 每年，要分型的患者数量将接近于 数字，尽管不太可能对所有患者进行研究，因为 那些非常年轻的人将不能捐赠足够数量的 检查所需的外周血样。 核心部分B用于支付#年的旅费和住房费。 来自每个参与机构的两名调查人员将前往 用于演示的中心站点(例如西雅图、丹佛或帕洛阿尔托) 结果比较，实验方案的修改，以及 决定每项研究的哪些方面需要重复、扩展、 或如有必要，停产。

The core for this program project is divided into two parts. Part A is HLA typing of all the diabetic patients to be studied in this research proposal--primarily at the Barbara Davis Diabetes Center at the University of Colorado, the Endocrinology Clinic at the University of Washington, and the Pediatric Endocrinology Clinic at the Medical of Wisconsin. In addition, a small number of patients will be studied at Stanford University, coming from the Pediatric Endocrinology Clinic of the Lucille Salter Children's Hospital. In this core, Dr. Henry Erlich will use polymerase chain reaction (PCR) based methods to type the HLA DR B1 and DQ B1 genotype of the patients to be studied in this study. Since these three major and one minor diabetes clinic between 250 to 300 new diabetes patients per year, the numbers of patients to be typed will approach that number, although it is not likely that all patients will be studied since those of very young age will not be able to donate sufficient amounts of peripheral blood for the studies required. Part B of the core is to provide for travel costs and housing costs for two investigators from each of the participating institutions to travel to a central site (e.g. Seattle, Denver, or Palo Alto) for presentation and comparison of results, modifications of experimental protocols, and decisions as to which aspects of each study need to be repeated, extended, or if necessary, discontinued.