Investigating the role of the deubiquitylating enzyme USP25 in cell cycle control

“BBSRC：Jessica布拉：BB/M01116 X/1“细胞是所有生物体的基本组成部分。蛋白质是分子机器，可以在细胞内发挥作用，调节细胞生长、细胞分裂和细胞死亡等过程。蛋白质种类繁多，它们都具有重要的生物学功能。蛋白质功能可以受到修饰的影响，例如一个或多个调节蛋白的连接。一种这样的调节蛋白被称为泛素。泛素连接的数量和3D方向对蛋白质的结构和功能有重要影响。例如，泛素修饰在蛋白质的及时和空间控制的靶向破坏中是重要的，这是在细胞分裂期间控制细胞中蛋白质功能的重要机制。蛋白质破坏的失调可导致不适当的细胞生长和疾病，如癌症。通过修饰了解蛋白质功能的正常过程对于增强我们对生物过程的理解以及鉴定可能具有治疗价值的药物非常重要。该项目将调查，具体地说，泛素修饰如何调节细胞的生长和分裂，通过专门消耗细胞中的蛋白质，控制细胞中泛素修饰的程度。具体来说，我们将研究蛋白质USP 25如何影响一种名为UbcH 10的蛋白质的功能，该蛋白质在控制细胞生长和分裂中非常重要，通过调节UbcH 10上的泛素修饰。因此，在该项目期间获得的数据将为蛋白质在细胞生长和分裂过程中的基本作用提供有价值的新见解，并帮助确定可能具有癌症治疗用途的药物。

"BBSRC: Jessica Bula: BB/ M01116X/1"The cell is the fundamental basal component of all living organisms. Proteins are molecular machines that can function inside cells to regulate processes such as cell growth, cell division and cell death. There is a diverse array of proteins, all of which possess important biological functions. Protein function can be affected by modification such as the attachment of one or more regulatory proteins. One such regulatory protein is called ubiquitin. The number and 3D-orientation of ubiquitin attachments can have important consequences for protein structure and function. For instance, ubiquitin modification is important in the timely and spatially-controlled targeting of proteins for destruction which is an important mechanism for controlling protein function in the cell during cell division. Dysregulation of protein destruction can lead to inappropriate cell growth and diseases such as cancer. Understanding the normal process of protein function by modification is important towards enhancing our understanding of biological processes, and towards the identification of drugs that might have therapeutic value. This project will investigate, specifically, how ubiquitin modification regulates cell growth and division by specifically depleting a protein from the cell that controls the extent of ubiquitin modifications in the cell. Specifically, we will investigate how the protein, USP25 affects the function of a protein called UbcH10, which is very important in controlling cell growth and division, through the modulation of ubiquitin modifications on UbcH10. As such, the data obtained during this project will provide valuable new insights into the fundamental role of proteins in the process of cell growth and division and help identify drugs that might possess cancer therapeutic use.