CORE--ELECTROPHYSIOLOGY

电生理学核心的目的是提供 为每个人提供集中的电生理服务 计划项目的组成部分。三种类型的专业化 将采取的措施：体内H反射措施 构成部分一、二、三和四，肌肉力量和疲劳性 组分I和II的原位检测和细胞内标记 在体外，第三和第四部分。核心设施将包括 两个相连的房间(一个350平方英尺)英国《金融时报》和一套175平方英尺的房子。英国《金融时报》) 中包含猫和大鼠运动/跑步机设置 较大的、分隔的房间，以及较小、 无隔间。这种邻接性将允许某些常见的 要在房间之间共享的设备，代表着 积蓄。用于活体H反射测试的放大器 (肌电图仪)和刺激器将需要，而在现场 记录肌肉力量和疲劳性将需要 增加了力传感器和脊柱立体定位仪 还有手术台。计算机数据分析(Supercope)和 存储(Neurodata)将在各个措施之间共享，而在 体外记录将需要一个细胞内放大器， 解剖显微镜、减振气台和电极 承运人。其中一些设备就在手边，只有一台左右 这一三重任务设施所需设备的第三部分是 被请求了。不同措施的集中记录 将允许跨组件的结果标准化， 允许跨范式和物种进行比较。

The purpose of the Electrophysiology Core will be to provide centralized electrophysiological services to each of the Components of the Program Project. Three types of specialized measures will be carried out: H-reflex measures in vivo in Components I, II, III and IV, muscle force and fatigability testing in situ in Components I and II, and intracellular labeling in vitro in Components III and IV. The Core Facility will consist of two contiguous rooms (one 350 sq. ft. and one 175 sq. ft.) containing the cat and rat exercise/treadmill set-ups in the larger, divided room, and a slice recording set-up In the smaller, undivided room. The contiguity will allow certain common pieces of equipment to be shared between rooms, representing significant savings. For in vivo H-reflex testing, amplifiers (electromyograms) and stimulators will be required, while in situ recording of muscle force and fatigability will require the addition of force transducers and a spinal stereotaxic instrument and surgical table. Computer data analysis (Superscope) and storage (Neurodata) will be shared across measures, while the in vitro recordings will require an intracellular amplifier, dissecting microscope, vibration damping air table and electrode carrier. Some of this equipment is on hand and only about one third of the equipment required for this triple-duty Facility is being requested. The centralized recording of different measures will allow standardization of results across Components, permitting comparisons across paradigms and species.