DEVELOPMENT OF PROBES FOR MULTIPHOTON & SEM MICROSCOPIES

集成多光子激发荧光和扫描 电子显微镜，使我们能够提炼细胞的信息 表面受体分布 在多光子显微镜中 允许减少散焦荧光，从而促进 观察细胞表面现象作为来自内部的信号 都被拒绝了 在扫描电子显微镜中， 可以在超微结构水平上验证标记物。 这一综合 当与冷冻技术结合时，这种方法特别有效。 仪器仪表的最新进展要求开发 在两种显微镜方法中都可以检测到的探针。 一步 在这个方向上，成功地与抗体缀合： a. 3 nm胶体金- 细胞表面和B.四甲基罗丹明-适用于 双光子激发 在这个项目中， 将进行衍生化。

Integrated multiphoton excitation fluorescence and scanning electron microscopies; allow us to refine the information on the cell surface receptor distribution. In multiphoton microscopy microscopy allows to reduce the out-of-focus fluorescence, thereby facilitating observations of cell surface phenomena as signals from the interior are rejected. In scanning electron microscopy, distribution of markers can be verified at the ultrastructural level. This integrated approach is particularly powerful when combined with cryotechniques. Recent advances in instrumentation call for the development of the probes which will be detectable in both methods of microscopy. A step in this direction was made with successful conjugation to antibodies: a. 3 nm colloidal gold - the smallest gold bead detectable with SEM on the cell surface and b. tetramethyl rhodamine - suitable for two-photon excitation. In this project other attempts of derivatizations will be pursued.