REGULATION OF CYTIDYLYLTRANSFERASE ACTIVITY

该实验室的工作重点是哺乳动物的调节 磷脂酰胆碱代谢，这是由酶调节 胆碱激酶和CTP：磷酸胆碱胞苷酰转移酶（CCT）。 我们假设细胞胆碱激酶活性是由 胆碱激酶同工酶在某些细胞中的选择性表达， 对某些信号的反应。 我们在隔离，表达， 分析胆碱激酶亚型的表达模式， 哺乳动物和线虫，C.优雅 此外，我们正研究 通过磷酸化和脂质调节CCT活性，并且已经被 研究这种酶的催化机制。 我们使用重组DNA 所有这些研究的技术，并要求使用GCG数据 碱基和序列分析软件来继续它们。

Work in this laboratory has focused on the regulation of mammalian phosphatidylcholine metabolism, which is regulated by the enzymes choline kinase and CTP: cholinephosphate cytidylyltransferase (CCT). We hypothesize that cellular choline kinase activity is modulated by the selective expression of choline kinase isozymes in certain cells and in response to certain signals. We are isolating, expressing, and analyzing the expression patterns of choline kinase isoforms from mammals and the nematode, C. elegans. In addition, we are studying the regulation of CCT activity by phosphorylation and lipids, and have been studying the catalytic mechanism of this enzyme. We use recombinant DNA techniques for all these studies, and require the use of the GCG data base and sequence analysis software to continue them.