SOCIAL REGULATION OF REPRODUCTIVE ENDOCRINOLOGY IN FEMALE MARMOSETS

目的：确定神经内分泌机制介导的 社会诱导性抑制从属雌性排卵 利用垂体柄-中位的推拉灌注的绒猴 主教大人 结果雌性普通绒猴的生殖能力为 受社会地位的制约。 社会地位低下的女性没有表现出 卵巢活动，垂体促性腺激素水平低。 我们 成功开发了一种推拉灌注技术， 清醒女性促性腺激素释放激素（GnRH）释放 绒猴同时采样促黄体生成激素（LH）， 颈静脉留置导管 由于下丘脑GnRH刺激 垂体促性腺激素LH的释放，我们最初 假设无排卵从属女性的低无环LH 这是由于GnRH释放受到抑制或减少。 但经过 成功地完成了30多个推拉灌注，然而，我们 发现（1）GnRH释放在下级雌性中不受抑制， (2)占主导地位的（中卵泡）和下属女性没有差异 反映GnRH释放的九个参数中的任何一个。 这表明 而不是戏剧性的下丘脑缺陷（通常与 低促性腺激素无排卵），社会抑制生育， 下级绒猴似乎是由一种新的神经内分泌介导的， 机制（S），也许在垂体水平。 未来 我们计划继续使用推拉灌注模式 在研究社会压抑生育的从属女性， 通过操纵神经内分泌因子， 垂体对GnRH的反应性，并通过检查 下丘脑-垂体对雌二醇刺激的反应 关键词 显性，不孕，下丘脑，垂体，GnRH，LH，推拉 灌注

OBJECTIVE: To identify neuroendocrine mechanisms mediating the socially-induced suppression of ovulation in subordinate female marmosets utilizing push-pull perfusion of the pituitary stalk-median eminence. RESULTS Female common marmoset reproductive capacity is regulated by social status. Socially subordinate females show no ovarian activity and have low levels of pituitary gonadotropins. We successfully developed a push-pull perfusion technique to characterize gonadotropin-releasing hormone (GnRH) release in conscious female marmosets while simultaneously sampling luteinizing hormone (LH) from an indwelling jugular catheter. Since hypothalamic GnRH stimulates the release of the pituitary gonadotropin LH, we initially hypothesized that low acyclic LH in anovulatory subordinate females resulted from suppressed or diminished GnRH release. However, after successfully completing more than 30 push-pull perfusions, however, we found (1) GnRH release is not suppressed in subordinate females, and (2) dominant (mid-follicular) and subordinate females do not differ across any of nine parameters reflecting GnRH release. This suggests that rather than a dramatic hypothalamic deficit (typically associated with hypogonadotropic anovulation), socially suppressed fertility in subordinate marmosets appears to be mediated by a novel neuroendocrine mechanism(s), perhaps at the level of the pituitary. FUTURE DIRECTIONS We plan to continue using the push-pull perfusion paradigm in studying socially suppressed fertility in subordinate female marmosets by manipulating neuroendocrine factors that may alter pituitary responsiveness to GnRH and by examining the hypothalamic-pituitary response to estradiol challenges. KEY WORDS dominance, infertility, hypothalamus, pituitary, GnRH, LH, push-pull perfusion