CONTROLLER DVMT TO MAINTAIN & VIEW SPECIMENS ABOVE/BELOW AMBIENT TEMPERATURE

在实验研究中，脊椎动物细胞需要保持 在33- 37 O C，有一些设备已经设计， 有效地完成这项任务。 然而，一些实验 条件要求电池的温度迅速且 可逆下降到100 ℃或更低。 例如， 选择拆卸，然后重新组装微管， 许多细胞是可逆的温度冲击。 去年，我们 建立了一个基于Peltier技术的样品温度控制器。 这个装置位于光学显微镜的载物台上， 玫瑰或灌注室，我们用于活细胞 实验 它能让培养物的温度 在不到90秒的时间内从37摄氏度迅速降至10摄氏度， 然后可将培养物从这些温度加热 于约 冷却时间减半 自从安装了这个装置 我们的GFP成像系统，许多人评论其实用性， 工作 因此，我们正在准备一份手稿， 在我们的工作过程中制造和使用。

During experimental studies vertebrate cells need to be maintained at 33-37O C and there are a number of devices that have been designed to effectively accomplish this task. However, some experimental conditions require that the temperature of the cell be rapidly and reversible dropped to 10O C or lower. For example, the method of choice for disassembling and then reassembling the microtubules in many cells is reversible temperature shocks. During the last year we built a specimen temperature controller based on Peltier technology. This device sits on the stage of a light microscope and surrounds the Rose or perfusion chambers that we used for live-cell experimentations. It allows the temperature of a culture to be rapidly dropped from 37 to 10oC in less than 90 seconds, and to 4oC in about 3 min. The culture can then be warmed from these temperatures in approx. half-the cooling time. Since installing this device on our GFP imaging system, many have commented on its utility to their work. As a result we are preparing a manuscript describing how it is fabricated and used during the course of our work.