IN SITU LASER ABLATION OF T LYMPHOCYTE ION CHANNELS

本研究项目正在研究生物物理性质和 电压门控性钾通道的亚基相互作用及其在心肌细胞中的作用 T淋巴细胞的生理学。具体地说，我们正在确定 不可逆性消融特定内源性K+对生理的影响 原代人类T细胞膜电位上的通道异构体 调节性体积减少，白细胞介素2分泌，细胞 增殖和凋亡(细胞程序性死亡)。此外，我们 正在确定功能通道和K+通道的周转率 用mRNA稳态水平来评估T细胞对消除 K+电流。我们选择使用分子激光消融 生色团激光辅助灭活(CALI)技术。

This research program is studying the biophysical properties and subunit interactions of voltage gated K+ channels and their roles in the physiology of T Lymphocytes. Specifically, we are determining the physiological impact of irreversibly ablating specific endogenous K+ channel isoforms in primary human T cells on membrane potential, regulatory volume decrease, interleukin-2 secretion, cell proliferation and apoptosis (programmed cell death). Additionally, we are determining turnover rates of functional channels and K+ channel mRNA steady-state levels to assess T cell responses to elimination of K+ current. We have chosen to use a molecular laser ablation technique, chromophore laser assisted inactivation (CALI).